DNA / RNA Extraction Buffers

Products | Kits, Reagents & Buffers | DNA / RNA Extraction Buffers

Severn TriReagent-RNA extraction, 200ml

Ref: 40-1111-02
Quantity:



Price:113.33
(Excluding VAT at 20%)



Poly Ethylene Glycol 8000, 100g

Ref: 30-76-01
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Price:23.31
(Excluding VAT at 20%)



30% Poly Ethylene Glycol 8000 solution, 100ml

Ref: 30-77-01
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Price:15.80
(Excluding VAT at 20%)



Phenol, saturated ph 6.6/7.9, 100ml

Ref: 40-1110-01
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Price:14.66
(Excluding VAT at 20%)



Phenol, saturated ph 6.6/7.9, 400ml

Ref: 40-1110-04
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Price:53.33
(Excluding VAT at 20%)



Phenol:Chloroform:Isoamyl Alcohol (25:24:1), 100ml

Ref: 40-1200-01
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Price:21.33
(Excluding VAT at 20%)



Phenol:Chloroform:Isoamyl Alcohol (25:24:1), 400ml

Ref: 40-1200-04
Quantity:



Price:36.00
(Excluding VAT at 20%)



Phenol:Chloroform:Isoamyl Alcohol (25:24:1), 200ml

Ref: 40-1200-20
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Price:21.50
(Excluding VAT at 20%)



Extraction Matrix 5ml

Ref: 20-8000
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Price:12.00
(Excluding VAT at 20%)



Elution Buffer, 50ml

Ref: 20-8700-05
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Price:6.80
(Excluding VAT at 20%)



L6 buffer, 150ml

Ref: 20-8600-15
Quantity:



Price:56.00
(Excluding VAT at 20%)



L2 buffer, 150ml

Ref: 20-8200-15
Quantity:



Price:53.00
(Excluding VAT at 20%)



DNA / RNA Extraction BuffersDNA / RNA Extraction Buffers
DNA and RNA are extracted using buffered redistilled water saturated phenol. The extraction of RNA is performed using phenol with a lower pH of 4.5. DNA is extracted at a higher pH of 6.0-8.0. Thistel Scientific offers re-distilled Water Saturated Phenol at different pH’s to accommodate the differences in extraction.

Phenol /Chloroform/Iso-amyl alcohol is also supplied to enable the partitioning of DNA and RNA precipitating proteins from solution. The protein precipitates at the inter-phase. At pH7.0 both DNA and RNA partition in the upper aqueous phase. Below pH7.0 DNA partitions with the protein fraction. For larger or bespoke pack sizes please contact our sales office. Phenol products are supplied in amber glass in order to reduce photo-oxidation. They are normally stored in a refrigerator at 4°C.

DNA/RNA extraction can be achieved without the use of Tris buffered Phenol. Using L2 & L6 Buffer in conjunction with an Extraction Matrix is a simple alternative to Phenol. Simply break down the cell walls and then follow the extraction protocols provided. The methods can be used on any cells including virus’ & bacteria.

L2 and L6 Buffers are designed for total extraction using chaotropic agents and an Extraction Matrix to capture the nucleic material from solution by solid phase binding. The extract is then eluted from the matrix using the Elution Buffer and then concentrated. It can also be used in conjunction with spin filter tubes.

Products | Kits, Reagents & Buffers | DNA / RNA Extraction Buffers