Electrophoresis Buffers

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Electrophoresis BuffersElectrophoresis Buffers
Several different electrophoresis buffers are available, the most popular containing Tris-acetate, Tris-borate and Trisphosphate. Historically, Tris-acetate has been the most commonly-used buffer. However, since its buffering capacity is comparatively low and it tends to become
exhausted during extended electrophoresis (the anode becoming alkaline and the cathode acidic), recirculation of buffer between the two reservoirs is recommended. This recirculation is unnecessary with both Tris-borate and Trisphosphate buffers, both of which give equally good
resolution of DNA fragments and have a significantly higher buffering capacity.

SDS is an effective protein denaturant which creates a net negative charge on the protein molecule. This allows the release of secondary structures in an aid to the separation of complex protein mixtures. Thus allowing accurate determination of the molecular weights of proteins when electrophoresed against known mw standard markers. Tris-glycine, buffers are all available with SDS already included, they are used primarily in proteomics for electrophoresis of protein molecules.

10x Tris Tricine, 1000ml

Ref: 20-6700-10
Quantity:



Price:80.80
(Excluding VAT at 20%)



20x Tris Taurine, 1000ml

Ref: 20-6900-10
Quantity:



Price:72.00
(Excluding VAT at 20%)



TBS 1x Solution, Sterile, 500ml

Ref: 20-7300-05
Quantity:



Price:12.60
(Excluding VAT at 20%)



TBS 1x Solution, Sterile, 1000ml

Ref: 20-7300-10
Quantity:



Price:16.00
(Excluding VAT at 20%)



TBS 10x solution, with w/v 0.5% Tween 20, 500ml

Ref: 20-7302-05
Quantity:



Price:15.40
(Excluding VAT at 20%)



TBS 10x solution, with w/v 0.5% Tween 20, 1000ml

Ref: 20-7302-10
Quantity:



Price:17.00
(Excluding VAT at 20%)



10x Tris-glycine SDS, 1000ml

Ref: 20-6400-10
Quantity:



Price:14.00
(Excluding VAT at 20%)



10x Tris-glycine, 1000ml

Ref: 20-6300-10
Quantity:



Price:13.50
(Excluding VAT at 20%)



10x TE, 1000ml (Clarify PH required with customer)

Ref: 20-6200-10
Quantity:



Price:8.00
(Excluding VAT at 20%)



1.5M Tris Buffer, pH (on request), sterile, 1000ml

Ref: 20-7900-10
Quantity:



Price:20.50
(Excluding VAT at 20%)



1M Tris Buffer, pH (on request), sterile, 1000ml

Ref: 20-7901-10
Quantity:



Price:15.70
(Excluding VAT at 20%)



10x TBE, 1000ml

Ref: 20-6000-10
Quantity:



Price:14.00
(Excluding VAT at 20%)



10x TAE, 1000ml

Ref: 20-6002-10
Quantity:



Price:10.00
(Excluding VAT at 20%)



Products | Kits, Reagents & Buffers | Electrophoresis Buffers