For gentle blotting of large
and temperature-sensitive proteins
For gentle blotting of large and temperature-sensitive
- Best suited for large proteins and proteins difficult
to blot as well as native enzymes
- Fast, efficient and reproducible transfer of proteins
- Effective cooling due to large-surface sidewall-integrated
- Simultaneous blotting of up to four 10 x 10 cm gels
During tankblotting proteins are transferred in a vertical
buffer tank between electrodes arranged on the sidewalls.
In the Tankblot a cooling jacket is integrated into the
sidewalls. This results in convective chilling by circulation:
the temperature is distributed more homogeneously than
with other designs (+/– 0.5°C at 6°C buffer temperature).
This special design also prevents formation of ion gradients.
The Tankblot is recommended particularly for blotting
of large molecules (> 100 resp. 200 kDa, depending on
the proteins´composition) or of proteins which are difficult
The Tankblot is the instrument of choice also for native
(temperature-sensitive) enzymes. By selecting an appropriate
buffer, transfer can be fast (between 30 min. and 4 h)
or, under exceptionally gentle conditions, overnight (ca.
12 h). Thanks to the big buffer volume of 1.2 liters transfer
times of more than 24 h can be realized, e. g. for gentle
blotting of very big proteins.