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Fastblot™ - Rapid Semi-Dry Blotting
  • Fast and homogeneous electrophoretic transfer of proteins
  • Choice of two types of models:
    • Maintenance-free metal electrodes
    • Carbon electrodes for metal-sensitive proteins
  • Cooling available
Electroblotting is an important method to transfer proteins from polyacrylamide gels to nitrocellulose or other carrier membranes.

Semi-dry blotting provides a means for both fast and homogenous transfer. In contrast to tank blotting little transfer buffer is required and transfer times are dramatically reduced. Additionally, with semi-dry blotting discontinuous buffer systems can be used, e.g. one cathode buffer and two different anode buffers, to gently blot smaller proteins or to transfer proteins of very different sizes evenly.

The first electrodes used for protein transfer were using graphite, which corroded easily. Biometra improved blotting electrodes by introducing a novel plasticized carbon-based and bio-inert material. Alternatively, now Biometra offers corrosion-free metal electrodes without pores. These electrodes consist of a platinum-coated titanium anode and a stainless steel cathode. Both the plasticized carbon electrodes and the metal electrodes can be used for higher currents so that blotting times are reduced to 10 to 30 minutes.

Large proteins (> 100 kDa) require longer transfer times, though. The heat that is generated by the extended transfer can be removed using the flow-through cooling system which is available as an option.
Biometra

Fast Blot

Fast Blot

By applying higher current, proteins with higher molecular weights can be blotted faster and more quantitatively. Even smaller proteins can be transferred faster from thick gels and gels with small pores when blotting with high current.

Nucleic acids can also be electro-blotted using the Tankblot systems. However, vacuum blotting is the method of choice for nucleic acids, e.g. with the Biometra Vacu-Blot.

All blotting instruments, including the above, need a powerful power supply, e.g. the versatile Biometra P25 Standard Power Pack, which can also be used for standard electrophoresis.

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