- Rapid Semi-Dry Blotting
Electroblotting is an important
method to transfer proteins from polyacrylamide gels to
nitrocellulose or other carrier membranes.
- Fast and homogeneous electrophoretic
transfer of proteins
- Choice of two types of models:
- Maintenance-free metal
- Carbon electrodes for
- Cooling available
Semi-dry blotting provides a means for both fast and homogenous
transfer. In contrast to tank blotting little transfer
buffer is required and transfer times are dramatically
reduced. Additionally, with semi-dry blotting discontinuous
buffer systems can be used, e.g. one cathode buffer and
two different anode buffers, to gently blot smaller proteins
or to transfer proteins of very different sizes evenly.
The first electrodes used for protein transfer were using
graphite, which corroded easily. Biometra improved blotting
electrodes by introducing a novel plasticized carbon-based
and bio-inert material. Alternatively, now Biometra offers
corrosion-free metal electrodes without pores. These electrodes
consist of a platinum-coated titanium anode and a stainless
steel cathode. Both the plasticized carbon electrodes
and the metal electrodes can be used for higher currents
so that blotting times are reduced to 10 to 30 minutes.
Large proteins (> 100 kDa) require longer transfer times,
though. The heat that is generated by the extended transfer
can be removed using the flow-through cooling system which
is available as an option.
By applying higher current, proteins with higher molecular
weights can be blotted faster and more quantitatively.
Even smaller proteins can be transferred faster from thick
gels and gels with small pores when blotting with high
Nucleic acids can also be electro-blotted using the Tankblot systems.
However, vacuum blotting is the method of choice for nucleic acids,
e.g. with the Biometra Vacu-Blot.
All blotting instruments, including the above, need a powerful power
supply, e.g. the versatile Biometra P25 Standard Power Pack, which
can also be used for standard electrophoresis.