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Temperature Gradient Gel Electrophoresis for analysis of DNA/RNA and proteins
  • precise temperature gradient by microprocessor control
  • highest reproducibility by up-to-date Peltier technology
  • system available in 2 formats
  • large gel format for long separation distance and high parallel sample throughput
  • accurate temperature control for applications at homogeneous temperatures possible
  • easy-to-program controller, suitable for advanced programs
Using the TGGE System of Biometra biomolecules can be separated according to migration behavior in a temperature gradient. This technique enables e.g. differentiation of DNA fragments with identical length but different sequence.

Unlike chemical or temporal temperature gradients the separation parameters can be controlled with high precision in the Biometra TGGE System. Thus, reproducible experimental conditions ensure reliable results.

Superior Know-How
Biometra has more than 10 years experience in mastering Peltier technology. This knowledge is the basis for the development of a high precision temperature gradient block for the TGGE System.


TGGE MAXI System: Long separation length and parallel analysis of multiple samples
The TGGE MAXI System offers a long separation length for analysis of complex samples. In addition a high number of samples can be analyzed in parallel. The TGGE MAXI system is the ideal instrument for the analysis of highly complex samples such as bacterial populations in 16S rRNA fingerprinting.
Unique Sensitivity
Sequencing of heterozygous samples after PCR amplification often leads to ambiguous results. Using the Biometra TGGE System it is possible to separate DNA fragment mixtures which differ in only one single position. Even mutations present on a single allele can be clearly detected.

In pre-screening of patient samples by molecular diagnostics the TGGE system offers a rapid and cost-effective method. Specific samples can be easily selected for sequencing. Based on the electrophoretic band pattern a differentiation between known and unknown mutations is often possible.
The heart of both TGGE systems is the Peltier temperature gradient block. The microprocessor control and regulation of the temperature gradient guarantees for highest reproducibility. Using this technology it is possible to perform any number of analysis under identical conditions. Thus, the TGGE system from Biometra is superior to systems based on chemical or temporal gradients.
  Wide Range of Applications
Currently the main fields of application are screening for mutations and analysis of microbial populations. However, the electrophoretic separation of complex biomolecules in a temperature gradient enables a broad range of applications e.g. in protein biochemistry.

Technical Specifications
Temperature range 5 °C 80 °C
Max. linear gradient 45 °C
Temp. field size 20 x 20 cm
Gel size 20 x 22 cm
Linear separation distance

16 cm
19 cm
Temp. uniformity +/- 0.5 °C
Temp. accuracy (at 80 °C) +/- 0.3 °C
Controller const. temperature
temperature gradient
electrophoresis control
Max. electrophoresis parameters 400 V, 500 mA, 30 W
Power supply external power supply