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The Convertible® Filtration Manifold System
THE CONVERTIBLE® Manifold is a dot/slot blotter designed for the immobilization of nucleic acids or proteins onto a membrane with the use of a vacuum source.
  • The patented design eliminates the need of screws or clamps for easy assembly.
  • Five interchangeable top plates and gaskets allow a variety of applications.
  • Leakproof seal provided by the same vacuum source that completes the filtration.
  • Top plates and gaskets indexed to the universal base plate to ensure proper fit.
  • Sample wells are arranged to match the dimensions of mul-tichannel pipettes.
  • Comes in a storage case to protect surfaces from scratches.
  • Apparatus components and precut membranes available separately.
  • Requires a minimum vacuum of 8 to 10 in of Hg.
Membrane pump MP86 is suitable for use with the Convertible®.

Biometra

The Convertible® Filtration Manifold System
Maximum Well Volumes
96-well 3mm Dot Top Plate
6-mm Dot Top Plate
450 ml
700 ml
48-well 1.0 x 4.0-mm Slot Top Plate
0.75 x 7.5-mm Slot Top Plate
750 ml
1,000 ml
20-well 1.5 x 70.0-mm Slot Top Plate 275 ml

Dimensions
Apparatus dimensions: (W x L x H) 17.9 x 19.1 x 3.8 cm
Base plate assembly: (W x L x H) 17.9 x 19.1 x 3.8 cm
Each top plate: (W x L x H) 17.9 x 13.6 x 1.7 cm

The Convertible® Filtration Manifold System The Convertible® Filtration Manifold System The Convertible® Filtration Manifold System The Convertible® Filtration Manifold System
96-well
dot
(3.0mm)
96-well
dot
(6.0mm)
48-well
slot
(0.75 x 7.5mm)
48-well
slot
(1.0 x 4.0mm)
Examples of chemiluminescent detection (above) and Western-blot processing (right) using THE CONVERTIBLE ‚ System. Above: Various dilutions of human DNA (1, 2.5, 5, 10, 25, 50, 100, and 250 ng) were filtered onto nylon membranes using the 96-well dot and 48-well slot formats. Right: Rat brain extract was electrophoresed using the Mini-V 8•10 Apparatus, blotted to nitrocellulose, and probed with various amounts of affinity-purified Anti-Protein Kinase C Isozyme-Specific Antibodies (e, d, and g, respectively) in THE CONVERTIBLE System. Enzyme activity was visualized using Goat-Anti-Rabbit IgG Alkaline-Phosphatase Conjugate and chromogenic substrates NBT and BCIP.
  MW (kDa)
97.4

The Convertible® Filtration Manifold System
68
  1.5 × 70.0mm-diameter slot format
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