agaroses
Severn Biotech's range of high-quality agaroses are all
prepared to exacting standards which ensure that the products
can be relied on time after time to produce optimal performance.
By altering critical characteristics, the agaroses have
been developed to meet the specific requirements of a
diverse range of applications. The main characteristics
are:
- Melting / gelling temperatures
- Gel strength
- Electroendosmosis (EEO)
Gels with low melting temperatures (below the temperature
at which DNA denatures) allow DNA to remain intact on
melting, thus enabling easy purification of nucleic acid
using b-agarase. SB Low Gel and Recovery will remain liquid
for several hours at temperatures compatible with many
in-gel enzymatic reactions and can be performed without
having to separate the nucleic acids from the media. High
gel strength is important if the application involves
handling and mechanical manipulation such as Southern
or Northern blotting. Finally the EEO (the movement of
non-charged molecules through a medium towards the cathode
during electrophoresis) of an agarose will determine the
mobility of certain macromolecules within the gel. Low
EEO values allow nucleic acids to migrate faster thereby
improving their resolution since migration is determined
by size only.
The agaroses within Severn Biotech's range can be used
for a wide variety of applications from nucleic acid separation
to immunoelectrophoresis and from tissue cell culture
to viral plaque assays. Recommended applications are indicated
with the description of each agarose type. |
AGAROSE
grades
SB Molecular Biology
This gel is an energetic all-rounder ideal for routine
analytical nucleic acid and protein electrophoresis. The
low EEO imparts a high electrophoretic mobility to macromolecules
enabling shorter electrophoretic runs with enhanced band
resolution. The high gel strength makes this agarose suitable
for Southern & Northern blotting techniques. Also ideal
for Ouchterlony & radial immunodiffusion (RID) techniques.
SB Fine Gel
SB Fine Gel is a standard melting temperature agarose
with a high gel strength designed for analytical electrophoresis.
It is ideal for fine resolutions of small DNA, RNA & PCR
fragments from 30 - 2, 000 bp and Southern & Northern
blotting techniques.
SB Low Gel
SB Low Gel is subjected to rigorous testing, including
several enzymatic performance measurements to ensure the
separated DNA can be used with common Molecular Biology
techniques such as cloning, PCR, restriction digestion,
ligation and labelling. It is ideal for separating and
resolving small PCR products (< 1 kb). DNA extraction
is unnecessary since cloning, PCR and sequencing reactions
can be carried out in the presence of the remelted agarose
gel.
The low gelling temperature allows heat labile substances to be incorporated
in the gel, thus making it suitable for culture and/or immobilisation
of bacteria, yeast & higher eukaryotic cells.
SB DNA Grade
SB DNA Grade has been developed to provide a low melting
and low gelling temperature agarose, with enhanced sieving
properties and optimal clarity. It is suitable for preparative
electrophoresis of nucleic acids and proteins and is also
ideal for the cloning of tissue culture cells and viral
plaque assays.
| Recommended
Applications |
| |
Mol
Biol |
Fine
Gel |
Low
Gel |
SB
DNA |
| RNA/DNA
Separation >1 kb |
X |
X |
X |
X |
| Resolution
of PCR Fragments |
X |
X |
X |
|
| Southern
& Northern Blots |
X |
X |
X |
|
| Fingerprinting |
X |
|
|
|
| DNA
Isolation |
X |
|
|
|
| Protein
Electrophoresis |
X |
|
|
|
| Glycoprotein
Electrophoresis |
|
|
|
|
| Immunoglobin
Electrophoresis |
|
|
|
|
| Immunoelectrophoresis |
|
|
|
|
| In-Gel
Manipulations I |
|
|
X |
|
| Immuno-assay
Techniques |
|
|
X |
|
| Tissue
Cell Culture |
|
|
X |
|
| Immobilisation
of Cells |
|
|
X |
|
| Preparative
DNA Fragment Isolation |
|
|
|
X |
| Tissue
Culture Cell Cloning |
|
|
|
X |
| Viral
Plaque Assays |
|
|
|
X |
|
