ENHANCED Autoradiography
- Direct alternative to fluorography
- A less hazardous low cost alternative
to fluorography
- Materials used in EA are environmentally
friendly, easy and safe to handle, store, transport and dispose
of
- Time saving process requires no expensive
capital equipment
- Generally non-destructive of the
sample which may be recovered
- Method of detection is film, which
gives highest possible resolution
Enhanced Autoradiography is an innovative process
which dramatically improves the detection efficiency of low energy
radionuclides, such as Tritium(3H), Carbon-14C (14C)
and Sulphur-35 (35S) on a flat matrix, e.g. PAGE/Agarose
blots, paper, polyester/aluminium backed TLC plates, etc. This significantly
reduces the time required to obtain experimental results from 8-10
weeks down to 3-4 days.
Wax supplied in 20ml syringes suitable for between 8-10 samples per
syringe. Comes complete with the 20x20cm Silicone Release Membranes
(SRM). |
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E.coli global protein change during
GroELS depletion. 2D gel labelled with 35S, electroblotted
to a PVDF membrane. The Enhanced Autoradiography process is
applied then laid down on film for 72 hours.
A similar image could not be obtained in a 2-3 week exposure
with the MD phosphor imager. Image courtesy of Dr J. Accord,
Edinburgh University |
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