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Bigneat PCR UV HEPA Cabinet

When undertaking PCR reactions it is important to protect against contamination by DNA from any source. The cabinet, reagents and equipment must be thoroughly decontaminated prior to a PCR process. UV irradiation is an efficient steriliser, breaking down DNA sequences so that replication cannot occur in a subsequent amplification process.

The necessary UV exposure times vary from as little as 5 minutes to a maximum of 30 minutes. UV is effectively utilised in both the Bigneat UV Sterilisation Cabinet and the Bigneat PCR Workstation.

The PCR Workstation additionally protects against contamination by bathing the PCR apparatus in sterile air and limiting risk of contamination from the operator’s arms, or from any equipment bought into the otherwise sterile environment.

A positive pressure ensures that there is no ingress of airborne contaminants from the surrounding laboratory environment.
  • Manufactured from the highest quality materials, which are highly resistant to corrosion and easy to clean.
  • Powerful UV tube lights are combined with carefully located optical reflectors to ensure uniform intensity distribution of the UV radiation throughout the cabinet.
  • Acrylic windows offer exceptionally clear all round vision, and do not allow transmission of UV.
  • Control system allows the choice of timed UV exposure from 5 – 30 minutes.
  • Door sensors switch off UV lamps if door flap is opened, ensuring operator safety.
  • Acrylic protects the operator from beta emissions from commonly used 32P labelled compounds.
  • Cabinet is supplied with handy corner shelves, which do not hinder the working area. Door flaps offer excellent access to the cabinet interior.
  • 15W white lights, operated from an external switch, illuminate cabinet interior.
  • Side panels include ports for cable access.
  • Auto speed increase on door opening maintains air velocity speed to 0.45 m/s at the workstation front
  • Electronic filter saturation indicator.
  • Fan noise level better than 50 d(B)a.

Fan housing and shelving: epoxy coated steel.

Support structure: anodised aluminium.

Windows: 10 mm thick acrylic. Base tray: PVC.

A HEPA filter system is used in this workstation.

Pre-filter has an efficiency of 90% to EFV grade EU7.

HEPA filter has an efficiency 99.997% for particulates as small as 0.3µm.