When undertaking PCR reactions it is important to
protect against contamination by DNA from any source.
The cabinet, reagents and equipment must be thoroughly
decontaminated prior to a PCR process. UV irradiation
is an efficient steriliser, breaking down DNA sequences
so that replication cannot occur in a subsequent
The necessary UV exposure times vary from as little
as 5 minutes to a maximum of 30 minutes. UV is effectively
utilised in both the Bigneat UV Sterilisation Cabinet
and the Bigneat PCR Workstation.
The PCR Workstation additionally protects against
contamination by bathing the PCR apparatus in sterile
air and limiting risk of contamination from the
operator’s arms, or from any equipment bought into
the otherwise sterile environment.
A positive pressure ensures that there is no ingress
of airborne contaminants from the surrounding laboratory
- Manufactured from the highest quality materials,
which are highly resistant to corrosion and
easy to clean.
- Powerful UV tube lights are combined with
carefully located optical reflectors to ensure
uniform intensity distribution of the UV radiation
throughout the cabinet.
- Acrylic windows offer exceptionally clear
all round vision, and do not allow transmission
- Control system allows the choice of timed
UV exposure from 5 – 30 minutes.
- Door sensors switch off UV lamps if door flap
is opened, ensuring operator safety.
- Acrylic protects the operator from beta emissions
from commonly used 32P labelled compounds.
- Cabinet is supplied with handy corner shelves,
which do not hinder the working area. Door flaps
offer excellent access to the cabinet interior.
- 15W white lights, operated from an external
switch, illuminate cabinet interior.
- Side panels include ports for cable access.
Fan housing and shelving: epoxy coated steel.
Support structure: anodised aluminium.
Windows: 10 mm thick acrylic. Base tray: PVC.