ibidi Pump System – Live Cell Media Perfusion and Defined Shear Stress

£10,713.00

Key Features

A pump system for the cultivation of, e.g. endothelial, cells under flow for simulation of blood vessels

  • Ideal simulation of various physiological conditions – continuous unidirectional, oscillating, and pulsatile flow
  • Compatible with the ibidi Heating Systems, all incubators, and incubated microscopes
  • Minimal mechanical stress, minimal amount of medium and supplement needed

System includes:

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ibidi Pump
I_1090X_Pump_System_part3.jpg
Notebook with PumpControl software
I_1090X_Pump_System_part4.jpg
Fluidic Unit
I_1090X_Pump_System_part4.jpg
Perfusion Set

Contact usfor a free demo of the ibidi Pump System.

SKU
10902
£10,713.00
SKU: 10902 Categories: ,
Special Requirements?

Description

Applications:

Components of the ibidi Pump System

ibidi Pump

  • Computer-controlled air pressure pump
  • Operates up to four parallel Fluidic Units per ibidi Pump

PumpControl Software

  • Easy setup of an automated flow control for flow assays
  • Internal calculation for flow rates, shear rates, and shear stress

Perfusion Set

  • Includes tubing and reservoirs to perform flow assays in combination with the Fluidic Unit
  • Designed for various flow rates and volumes (see table below)

Fluidic Unit

  • Flow-switching device with electrically controlled valves
  • Equipped with a holder for one Perfusion Set (10 ml) and connected μ-Slide(s)
  • For operation in the cell culture incubator
  • Optional: Reservoir Holder for 2 ml and 50 ml Perfusion Sets

Optional: Fluidic Unit Quad

  • Combines four Fluidic Units into one system
  • Operated by a single ibidi Pump

Optional: Heating Insert Adapter for Perfusion Assay

Technical Features:

  • Operates up to four parallel Fluidic Units per ibidi Pump
  • Flow characteristics: all flow types are laminar
  • Some examples:
    • Unidirectional flow simulates veins or small arteries
    • Oscillating flow simulates disturbed conditions at vessel branchings or valves
    • Pulsatile flow simulates arterial conditions
  • Flow rate using ibidi µ-Slides: 0.1 – 40 ml/min
  • Shear stress using ibidi µ-Slides: 0.2 – 80 dyn s/cm2
  • Working volume: 2.5 ml / 12 ml / 50 ml
  • Suitable for all µ-Slides with Luer adapters
  • Also suitable for home-made flow chambers
  • Compatible with all incubators
  • Software-controlled flow rates and shear stress

System Overview:

The ibidi Perfusion System Perfectly Reflects the Natural Environment of Cells Under Flow Conditions

The ibidi Pump System consists of two main components: The ibidi Pump (computer controlled air pump, placed outside the incubator) and the Fluidic Unit (holder for cell media reservoirs and μ-Slide, tubing, and electrically controlled valve set, placed inside the incubator). By using this “split” approach, the closed circulation of the medium can be assembled separately in the laminar flow hood and transferred to the microscope or the incubator, without compromising the sterility of the system. The open architecture using the Luer connectors allows the use of any kind of flow devices. The PumpControl software controls the pressure, and subsequently the shear stress acting on the cells, and provides the possibility to program complex flow patterns.

Placing the Fluidic Unit inside a large-size microscope incubator allows for running long-term perfusion assays directly on the microscope. A defined temperature and CO2 concentration is assured over the whole perfusion, which is ncecesary for avoiding air bubbles. For short-term experiments, the usage of a small incubator, hosting the Fluidic Unit, next to the microscope is sufficient.

Principle of Unidirectional Flow

Applying pressured air to the reservoirs of the Fluidic Unit moves the medium and generates the flow in the μ-Slide channels. In order to avoid wasting any medium, the liquid is pumped back and forth between reservoir A and reservoir B by switching the valve V1.

To assure a unidirectional flow in the channel, the other valve V2 is switched simultaneously. As the tubing parts inserted in the valve V2 are criss-crossed, the medium is always directed to the same end of the channel slide.

Example of a Perfusion Set-up on a Microscope

Ideal Setup inside a large-size microscope incubator. In combination with the ibidi Heating & Incubation System for short-term experiments.

The ibidi Pump System Generates Minimal Mechanical Stress

Application Examples:

rAV CMV-LifeAct-TagRFP-transduced HUVEC, cultivated under 20 dyn / cm² in µ-Slide I 0.4 Luer

HUVEC, cultivated over 7 days at 10 dyn / cm². VE-cadherins are stained in green, cell nuclei are stained in blue.

Rolling and adhesion of polymorpho-
nuclear leukocytes on LPS-stimu-
lated cerebrovascular endothelial cells in µ-Slide VI 0.4 at 1 dyn / cm².
(Courtesy of G. Cepinskas, Ontario)

Suitable µ-Slides

The following ibidi µ-Slides can be attached to the ibidi Pump System:


NOTE:
Custom specific slides can also be used when utilizing the Luer adapters.

Perfusion Sets and µ-Slide Selection

Overview:

Blue
(ID 0.8 mm)
Red
(ID 1.6 mm)
White
(ID 0.8 mm)
Yellow
(ID 1
/Green
.6 mm)
Yellow
(ID 0.5 mm)
Black
(ID .5 mm)
Low shear stress X X
High shear stress X X X X
Long tubes for
microscopy (50 cm)
X X X
Working volume (ml) 12 12 12 12 2.5 2.5
Standard reservoir* (ml) 10 10 10 10 2 2

* By changing the reservoir, different working volumes can be achieved. Reservoirs are available with a syringe volume of 2, 12, and 50 ml.

Details:

NOTE:The given shear stress ranges are calculated with the viscosity of standard medium with 10%FCS at 37°C (0.0072 dyn s/cm²).

 

Perfusion Set blue (#10961)
Inner diameter: 0.8 mm Tube length: 15 cm Viscosity 0.0072 dyn s/cm²
Total working volume: 11.3 ml Dead volume of tubing: 0.5 ml
Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 0.58 5.4 2.2 19.9
µ-Slide I 0.4 Luer 2.5 100 0.83 7.0 0.79 6.7
µ-Slide I 0.6 Luer 2.5 150 0.89 7.1 0.38 3.1
µ-Slide I 0.8 Luer 2.5 200 0.89 7.1 0.22 1.8
µ-Slide VI 0.4 0.6 30 0.90 7.1 1.1 9.0
µ-Slide y-shaped 2.8 110 0.86 7.2 1.4 11.8
without slide (75)* 0.86 6.9

* Dead volume of Female Luer Lock Coupler

 

Perfusion Set red (#10962)
Inner diameter: 1.6 mm Tube length: 15 cm Viscosity 0.0072 dyn s/cm²
Total working volume: 12.3 ml Dead volume of tubing: 1.5 ml
Growth
area [cm²]
Channel
volume [µl]
Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 2.0 22.6 7.5 83.3
µ-Slide I 0.4 Luer 2.5 100 4.2 39.2 4.0 37.1
µ-Slide I 0.6 Luer 2.5 150 4.4 41.3 1.9 17.9
µ-Slide I 0.8 Luer 2.5 200 4.4 41.5 1.1 10.4
µ-Slide VI 0.4 0.6 30 4.4 41.7 5.6 52.9
µ-Slide y-shaped 2.8 110 4.1 37.9 6.8 62.1
without slide (75)* 4.5 42.3

* Dead volume of Female Luer Lock Coupler

 

Perfusion Set white (#10963)
Inner diameter: 0.8 mm Tube length: 50 cm Viscosity 0.0072 dyn s/cm²
Total working volume: 11.7 ml Dead volume of tubing: 0.9 ml
Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 0.31 2.7 1.1 9.9
µ-Slide I 0.4 Luer 2.5 100 0.35 3.1 0.33 2.9
µ-Slide I 0.6 Luer 2.5 150 0.36 3.1 0.16 1.4
µ-Slide I 0.8 Luer 2.5 200 0.36 3.1 0.09 0.78
µ-Slide VI 0.4 0.6 30 0.33 3.1 0.42 3.9
µ-Slide y-shaped 2.8 110 0.36 3.1 0.59 5.0
without slide (75)* 0.39 3.2

* Dead volume of Female Luer Lock Coupler

 

Perfusion Set yellow/green (#10964)
Inner diameter: 1.6 mm Tube length: 50 cm Viscosity 0.0072 dyn s/cm²
Total working volume: 13.6 ml Dead volume of tubing: 2.8 ml
Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide I 0.2 Luer 2.5 50 1.6 20.1 6.1 74.1
µ-Slide I 0.4 Luer 2.5 100 3.1 30.6 2.9 29.0
µ-Slide I 0.6 Luer 2.5 150 3.2 32.4 1.4 14.0
µ-Slide I 0.8 Luer 2.5 200 3.4 35.7 0.8 8.9
µ-Slide VI 0.4 0.6 30 3.3 34.2 4.2 43.3
µ-Slide y-shaped 2.8 110 3.1 31.4 5.1 51.4
without slide (75)* 3.9 42.5

* Dead volume of Female Luer Lock Coupler

 

Perfusion Set yellow (#10965)*
Inner diameter: 0.5 mm Tube length: 15 cm Viscosity 0.0072 dyn s/cm²
Total working volume: 2.5 ml Dead volume of tubing: 0.5 ml
Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide VI 0.1 0.17 1.7 0.17 0.74 12.8 56.7

* Perfusion Set only works with PumpControl version 1.5.0 or higher and ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.

 

Perfusion Set black (#10966)*
Inner diameter: 0.5 mm Tube length: 50 cm Viscosity 0.0072 dyn s/cm²
Total working volume: 2.7 ml Dead volume of tubing: 0.7 ml
Growth area [cm²] Channel volume [µl] Flow rate [ml/min] Shear stress [dyn/cm²]
MIN MAX MIN MAX
µ-Slide VI 0.1 0.17 1.7 0.07 0.33 5.4 25.7

* Perfusion Set only works with PumpControl version 1.5.0 or higher and ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.