Primary T cells were isolated from the spleen of a LifeAct mouse. An under-agarose assay (UA-assay) was performed to analyze chemotaxis and chemokinesis. Fluorescent live cell images illustrate the movement of the LifeAct-stained actin cytoskeleton.
J. Riedl, K. C. Flynn, A. Raducanu, F. Gärtner, G. Beck, M. Bösl, … R. Wedlich-Söldner. Lifeact mice for studying F-actin dynamics. Nature Methods, 2010, 10.1038/nmeth0310-168
B. Heit and P. Kubes. Measuring Chemotaxis and Chemokinesis: The Under-Agarose Cell Migration Assay. Science Signaling, 2003, 10.1126/stke.2003.170.pl5