Cell Culture Under Flow

Experimental Workflow

Experimental workflow

Cell Seeding Into Channel Slides

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Procedure

The cell suspension is filled into the channel slide. After cell attachment, the flow experiment can be started. ibidi provides channel slides with different channel heights and geometries, which are suitable for various experimental conditions. For details, please refer to the chapter “The Optimal Perfusion Set and Slide for Your Application“.

ibidi Products for This Step

Flow Conditioning of Adherent Cells

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Procedure

After connecting the channel slide to the ibidi Pump System, the flow can be applied. Under flow, the cell culture medium is continuously pumped through the channel slide. This results in shear stress that influences the cell behavior and physiology. The flow type, flow rate, and the assay duration depend on the experimental setup. Find more details about how to plan the optimal experimental setup here.

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Staining and Image Acquisition

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Procedure

Using live cell microscopy, images or videos of the flow-conditioned cell layer can be directly acquired within the channel slide. Further, immunofluorescence stainings and subsequent imaging can be performed directly in the channel slide.

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Quantitative and Statistical Analysis

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Procedure

After the flow experiment, the cells can be easily analyzed using, e.g., Western blot, qPCR, FACS, and more downstream methods.