µ-Dish 35 mm, high Grid-50 Glass Bottom

£215.00

Key Features

A 35 mm imaging dish with a glass bottom and an imprinted 50 µm cell location grid

  • Ideal for locating cells or cell clusters
  • Easy cell counting per defined area
  • Glass coverslip for TIRF and single molecule applications

SKU
81148
£215.00
Special Requirements?

Description

Applications:

  • Locating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., when calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection

Technical Features

  • Grid with 50 µm µm repeat distance
  • Lettered and numbered 4 x 400 squares (A-U; 1-20)
  • Grid and cells in one focal plane
  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
  • Lid with lock position, which minimizes evaporation
  • May require coating to promote cell attachment
  • Also available in a Grid-500 version with ibidi Polymer Coverslip Dish 35 mm, high Grid-500 or µ-Slide 8 Well Grid-500

Specifications:

Grid repeat distance50 µm
Ø µ-Dish35 mm
Volume2 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Ø observation area21 mm
Height with / without lid14/12 mm
BottomGlass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Layout Grid-50

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
AutofluorescenceLowLow
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
BreakableNoYes
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately

    Additional information

    Material

    Glass

    Quantity

    30