µ-Dish 35 mm, low Grid-500


Key Features

A 35 mm imaging dish with an ibidi Polymer Coverslip bottom, low walls, and an imprinted 500 µm cell location grid

  • Ideal for locating cells or cell clusters
  • Unique ibidi Polymer Coverslip combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid

Special Requirements?



  • Relocating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., for calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection
  • Following axon growth on a defined scale bar

Technical Features

  • Standard format dish with a 35 mm diameter
  • Grid with a 500 µm repeat distance
  • Lettered and numbered fields (A-U; 1-20)
  • Also available with a glass bottom: µ-Slide 35 mm, high glass bottom with Grid-50 or with Grid-500
  • Ideal cell growth conditions provided by the ibiTreat tissue culture treated surface
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • Lid with lock position, which minimizes evaporation
  • Grid-500 also available in a µ-Slide 8 Well version


Grid repeat distance500 µm
Ø µ-Dish35 mm
Volume0.8 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Ø observation area21 mm
Height with lid9 mm
Bottom: ibidi Polymer Coverslip

Layout Grid-500

Lid with Lock Position

The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required, i.e., outside incubators, non humidified microscopy stages, etc.

ibidi µ-Dish Family: Versions and Variations

High walls:
Used for all standard applications

Low walls:
Provide greater cell access (useful for micromanipulation)

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately

    Additional information


    Uncoated, ibiTreat


    ibidi Polymer