µ-Plate Angiogenesis 96 Well

£406.00

Key Features

A µ-Plate 96 Well used to investigate angiogenesis in high throughput tube formation assays

  • Fully SBS (Society for Biomolecular Screening) and robotics compatible screening plate
  • Brilliant visualization without meniscus formation, and with all cells in one focal plane
  • Cost-effective high throughput experiments, requiring only 10 µl of gel per well

SKU
89646
£406.00
SKU: 89646 Categories: ,
Special Requirements?

Description

Applications:

Technical Features:

Specifications:

Number of wells96
Length / Width127.7 / 85.5 mm
Heigh with / without lid16.6 / 14.4 mm
Well-to-well distance9.0 mm
Volume inner wells10 µl
Ø Inner wells4 mm
Volume upper wells70 µl
Ø Upper wells5 mm
Growth area per inner well0.125 cm²
Coating area per inner well0.23 cm²
Well clearance1.0 mm
Bottom: ibidi Polymer Coverslip

Angiogenesis Assays in vitro

Cells on gel matrices can be used to monitor their ability to form new vascular structures. Tube formation assays are carried out by seeding single cells onto the gel matrix and observing characteristic patterns. To carry out sprouting assays, either spheroids or pieces of tissue, e.g., from the aorta, are placed onto the gel matrix. Both these types of assays require that the underlying gel layer be of uniform thickness.

µ-Plate Angiogenesis 96 well vs. Standard 96 well Plate


µ-Plate Angiogenesis
96 Well

  1. Planar air-liquid interface:
    good phase contrast all over the observation area
  2. Planar gel surface:
    all cells are in one optical plane

Volume of Matrigel: 10 µl


Standard well

  1. Meniscus on air-liquid interface:
    poor phase contrast in most of the observation area
  2. Mensicus on the gel surface: not possible to focus on all cells simultaneously

Volume of Matrigel: 100 µl

Technical Drawing

Technical drawings and details are available in the Instructions (PDF).

Sam Noppen, Rega Institute for Medical Research, Leuven, Belgium

“I used the ibidi µ-Plate Angiogenesis 96 Well for bioluminescence measurements. The plate performed exceptionally well for this specific application, as it reduced my sample volume drastically and the optical quality was just superb.”

Sam Noppen
Rega Institute for Medical Research, KU Leuven
Leuven
Belgium

https://www.kuleuven.be/rega/cmt/

Additional information

Quantity

15