µ-Slide 18 Well


Key Features

  • Pack of 15
  • All-in-one 18 well chamber slide for cost-effective experiments with small cell numbers and low reagent volumes
  • Ideal for high-resolution imaging through the No. 1.5 polymer coverslip bottom with the highest optical quality
  • Suitable for various microscopy techniques (e.g., DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, and LSFM)

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A chambered coverslip with 18 wells for cell culture, immunofluorescence, and high-end microscopy.

The µ-Slide 18 Well is a versatile live cell imaging chamber slide, suitable for a range of imaging applications. The split chamber design allows for cost effective experiments with small cell numbers and low reagent volume. Combined with the ibidi #1.5 polymer coverslip bottom, this slide gives the highest optical quality for high resolution fluorescence microscopy.

Applications Include:

  • Cultivation and high-resolution microscopy of cells
  • Immunofluorescence staining and fluorescence microscopy of living and fixed cells
  • Live cell imaging over extended time periods
  • Transfection assays
  • Differential interference contrast (DIC) microscopy when used with a DIC lid

Technical Features

  • Chambered coverslip with 18 independent wells and a non-removable polymer coverslip-bottom
  • ibiTreat (tissue culture-treated) surface for optimal cell adhesion
  • Imaging chamber slide with excellent optical quality for high-end microscopy
  • Closely fitting lid for low evaporation
  • Individual well walls for minimizing well-to-well crosstalk and contaminations
  • Compatible with staining and fixation solutions
  • Biocompatible plastic material—no glue, no leaking
  • Also available as an adhesive version without a bottom: sticky-Slide 18 Well
  • Also available with a Glass Coverslip Bottom: µ-Slide 18 Well Glass Bottom for special microscopic applications

Principles of the µ-Slide 18 Well

The Coverslip Bottom

The µ-Slide 18 Well comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 18 Well without any bottom, or and as the µ-Slide 18 Well Glass Bottom for special microscopic applications.

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Outer dimensions (w x l)25.5 x 75.5 mm²
Number of wells18
Dimensions of wells (w x l x h)5.7 x 6.1 x 6.8 mm³
Volume per well100 µl
Height with/without lid8.2/6.8 mm
Growth area per well0.34 cm²
Coating area per well1.15 cm²
Bottom: ibidi Polymer Coverslip


Instructions u-Slide 18 Well

Application Notes

AN 08 – Cell Culture Coating
AN 58 Immonofluorescence with u-Slide 18 Well

Application Guides

2020 Immunofluorescence Application Guide
μ-Slide 18 well