µ-Slide 8 Well Glass Bottom

£160.00£834.00

Key Features

Open µ-Slide with 8 wells with a glass bottom, suitable for use in TIRF and single molecule applications

  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

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Description

Applications:

  • Cultivation and microscopy of cell cultures
  • TIRF and single molecule applications

Technical Features

Specifications:

Number of wells8
Dimensions of wells
(w x l x h) in mm
9.4 x 10.7 x 6.8
Volume per well300 µl
Total height with lid8 mm
Growth area per well1.0 cm²
Coating area per well2.20 cm²
Bottom:Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
AutofluorescenceLowLow
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
BreakableNoYes
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately

Jessica Davis, University College Dublin, Ireland

“I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment — I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”

Jessica Davis
University College Dublin
Ireland

https://www.ucd.ie/

    Additional information

    Quantity

    15, 90

    Material

    Glass