µ-Slide I Luer 3D

SKU: IB-87171 UNSPSC: 41122111 Manufacturer Part No: Array Pack Size: 15
  • Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
  • Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
  • Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells
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A µ-Slide with one channel and three wells for culturing cells on a 3D gel matrix with defined flow

Applications

  • Culture of adherent cells on a 3D gel matrix under flow conditions
  • Transendothelial migration studies under flow conditions
  • Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
  • Simulation of blood vessels with endothelial cell culture on a soft substrate
  • Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
  • Apical-basal cell polarity assays
  • Cell barrier model assays with apical-basal gradients
  • Cell boundary assays without filters or porous membranes
  • Rolling and adhesion assays using leukocytes
  • Inflammation studies
  • Transport studies

Technical Features:

  • Can create low or high shear stress in the 0.6 mm channel
  • Initial open well format allows for easy sample preparation, then is simply closed with a coverslip for the application of flow
  • The well design corrects meniscus formation and allows for superb cell visualization in one focal plane
  • Can be used with all ECM hydrogels
    (e.g., Collagen Type I, Matrigel, Fibrinogen)
  • Contains Luer adapters for easy tube and pump connection (e.g., to the ibidi Pump System)
  • ibidi Polymer Coverslip on top and bottom for high-resolution light microscopy
  • Partially compatible with upright microscopy
  • Defined shear stress levels
  • Specifications

    Outer dimensions (w x l) 25.5 x 75.5 mm²
    Number of wells 3
    Volume of wells 16 μl
    Well dimensions 5.4 mm x 4.0 mm
    Well height (without channel) 0.8 mm
    Growth area per well 0.21 cm²
    Coating area per well 0.34 cm²
    Channel width 5.0 mm
    Channel volume (without wells) 150 μl
    Channel height (without well) 0.6 mm
    Adapters Female Luer
    Volume per reservoir 60 μl
    Top cover: ibidi Polymer Coverslip
    Bottom: ibidi Polymer Coverslip

    Specifications

    Outer dimensions (w x l) 25.5 x 75.5 mm²
    Number of wells 3
    Volume of wells 16 μl
    Well dimensions 5.4 mm x 4.0 mm
    Well height (without channel) 0.8 mm
    Growth area per well 0.21 cm²
    Coating area per well 0.34 cm²
    Channel width 5.0 mm
    Channel volume (without wells) 150 μl
    Channel height (without well) 0.6 mm
    Adapters Female Luer
    Volume per reservoir 60 μl
    Top cover: ibidi Polymer Coverslip
    Bottom: ibidi Polymer Coverslip
    Variant
    Pack/Size
    Ordering
    Price
    Qty
    slide-i-luer-3d
    µ-Slide I Luer 3D Uncoated: #1.5 polymer coverslip,hydrohobic, sterilized
    15 / Pack

    SKU: IB-87171

    Manufacturer Part No: Array

    UNSPSC Code: 41122111

    Price: £243.37 ex. VAT
    slide-i-luer-3d
    µ-Slide I Luer 3D ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
    15 / Pack

    SKU: IB-87176

    Manufacturer Part No: 87176

    UNSPSC Code: 41122111

    Price: £243.37 ex. VAT

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