µ-Slide Membrane ibiPore Flow Kit

Key Features

  • Brilliant optical quality through the thin, porous glass membrane
  • Full fluidic access to the apical and basal sides of adherent cells in numerous applications
  • Different pore sizes available for various cell types
  • Suitable for the establishment of lung models with air-liquid interface (ALI)
  • Including pipet tips, petri dishes, and syringes

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SKU: µ-Slide Membrane ibiPore Flow Kit Categories: , , , , , , ,
Special Requirements?

Description

A µ-Slide with a porous glass membrane for transmigration and transport studies under both static and flow conditions

  • Brilliant optical quality through the thin, porous glass membrane
  • Full fluidic access to the apical and basal sides of adherent cells in numerous applications
  • Different pore sizes available for various cell types
  • Suitable for the establishment of lung models with air-liquid interface (ALI)

Applications

  • Trans-endothelial migration under flow conditions
  • Co-cultivation of cell layers and transport in 2D or in a 3D gel matrix
  • Apical-basal cell polarity assays
  • Skin and lung models with liquid-air interface
  • Cell barrier model assays with apical-basal gradients
  • Cell migration assays based on filters and porous membranes

Technical Features

  • SiMPore’s G-FLAT™ Microporous Glass Membranes
  • Cross-channel structure with a porous optical membrane in between
  • Excellent optical properties comparable to a glass coverslip
  • Pore sizes 0.5 µm, 3 µm, 5 µm, or 8 µm available
  • Membrane thickness 0.3 µm (300 nm)
  • For use with objective lenses with a working distance >0.5 mm
  • Fully compatible with the ibidi Pump System
  • Defined shear stress and shear rate levels
    (For details see Application Note 11)

Specifications

Total coating area4.50 cm²
Bottomibidi Polymer Coverslip
Main Channel (Lower Channel)
AccessLuer port, accessible with female Luers
Volume50 µl
Height0.4 mm
Length25 mm
Width5 mm
Growth area1.25 cm²
  
Upper Channel
AccessReservoir port, accessible with 20/200 µl pipet tips
Volume55 µl
Height over membrane1.3 mm
 
 
ibiPore Membrane
MaterialSiO2 (glass)
Thickness0.3 µm (300 nm)
Membrane size2 mm x 2 mm
Porous area1.77 mm x 1.84 mm
Restrictions for objective lensesWorking distance >0.5 mm
Pore layoutHexagonal spacing
Available Variations

Pore size
0.5 µm
3 µm
5 µm
8 µm
Porosity
20%
5%
5%
5%
Pore-to-pore distance
1 µm
12 µm
20 µm
32 µm