µ-Slide 15 Well 3D Glass Bottom

A chambered coverslip with 15 wells and a glass bottom for 3D cell culture, tube formation assays, and immunofluorescence

• Brilliant cell visualization without gel meniscus formation
• Cost-effective experiments, requiring only 10 µl of gel per well
• For a broad range of gels (e.g., Matrigel^®, collagen, and agarose)

Applications

• Tube formation assays
• 3D cell culture
• Immunofluorescence staining
• Sprouting assays
• Live cell imaging of attached cells
• TIRF and super-resolution microscopy

Technical Features

• Standard slide format
• Bottom made from D 263 M Schott glass, No. 1.5H
  (170 +/- 5 µm)
• May require coating to promote cell attachment
• Closely fitting lid to reduce evaporation
• 4 mm well within a 5 mm well
• Geometry enables pipetting of a homogeneous 0.8 mm thick gel layer*
• Provides homogeneous cell growth
• Compatible with staining and fixation
• Excellent optical properties for microscopy
• Compatible with multi-channel pipettes
• Suitable for use with various types of gels* (e.g., Matrigel^®, collagen, and agarose)
• Also available with an ibidi Polymer Coverslip bottom: µ-Slide 15 Well 3D
• Also available in a 96 well format: µ-Plate 96 Well 3D

*The gel matrix is not included with this product.

Every Cell in Focus:
The Principle of the µ-Slide 15 Well 3D Glass Bottom

The μ-Slide 15 Well 3D Glass Bottom has a specialized geometry for the easy, convenient, and reproducible conduction of tube formation assays. It is also ideal for sprouting assays, immunofluorescence staining, and general 3D cell culture.

After the gel has been pipetted into the inner well and given time to solidify, the cells can be seeded on top of it for tube formation analysis. Due to the “well-in-a-well” technology, the amount of gel needed is reduced to only 10 μl per well, which is 10% of the amount used in regular multiwell plates.

Further, no gel meniscus is formed. This ensures the formation of a uniformly thick gel matrix on which all cells are in one optical plane, creating reproducible cell culture conditions. The µ-Slide 15 Well 3D Glass Bottom can be used with all common hydrogel matrices, such as Matrigel^®, collagen gels, agarose gels, and hyaluronic acid gels.

With its 15 wells, the µ-Slide 15 Well 3D Glass Bottom is designed for low throughput assays. For large scale applications, ibidi provides the μ-Plate 96 Well 3D.