|Number of wells||4|
|Outer dimensions||Ø 17 mm|
|Volume per well||110 µl|
|Growth area per well||0.35 cm²|
|Coating area per well||1.23 cm²|
|Width of cell free gap|
|– Two cell fronts||500 µm +/- 50 µm|
|– Four cell fronts (center)||1000 µm +/- 100 µm|
|Bottom||No bottom – sticky underside|
Principle for Wound Healing and Migration Assays
cell seeding and attachment
remove insert after cultivation
overlay patches with culture medium
Wound Healing / Migration
The Culture-Insert 4 Well allows for wound healing and migration assays with four cell-free gaps with a 0.5 mm gap width, and one center area with a 1 mm diagonal distance. Compared to the 2 well version, more cells can be analyzed and a higher throughput is realized. Two cell-free gaps create a technical replicate in the very same well.
Two Conditions of Wound Healing / Migration
With the Culture-Insert 4 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).
Four Conditions for Co-Cultivation / Invasion
By seeding up to four different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.
Invasion and co-cultivated cells can be imaged by using high-resolution fluorescence microscopy.
Visualization of Cell Migration
Performing a Wound Healing Assay