3-D Life 10x CB-Buffer (pH 5.5) is a component of the 3-D Life Hydrogel system. It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life Hydrogel system. It is used for the formation of fast gelling 3-D Life Hydrogels where it provides a cell environment at pH 5.5, which is compatible with most cell types. In conjunction with 3-D Life 10x CB-Buffer (pH 7.2) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels.For instructions, please consult the General Protocol GP-1 “Preparation of 3-D Life Fast Gelling Hydrogels” and the 3-D Life Hydrogels User Guide.
Used with other components of the 3-D Life Hydrogel System to establish 3-D cell cultures.
Sufficient for at least 6 ml 3-D Life Hydrogel.
|Material||Quantity||Concentration of reactive groups|
|10x CB (pH 5.5)||600 µl||n.a.|