3-D Life 10x CB-Buffer (pH 7.2) is a component of the 3-D Life Hydrogel system. It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life Hydrogel system. It is used for the formation of slow gelling 3-D Life Hydrogels where it provides a cell compatible environment at pH 7.2. In conjunction with 3-D Life 10x CB-Buffer (pH 5.5) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels.For instructions, please consult the General Protocol GP-2 “Preparation of 3-D Life Slow Gelling Hydrogels” and the 3-D Life Hydrogels User Guide.
Used with other components of the 3-D Life Hydrogel System to establish 3-D cell cultures.
Sufficient for at least 6 ml 3-D Life Hydrogel
|Material||Quantity||Concentration of reactive groups|
|10x CB (pH 7.2)||600 µl||n.a.|