The 3-D Life PVA-PEG Hydrogel Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are polymeric
Mal-PVA and PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.
Prior to the crosslinking step, peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.
For more information and instructions, please consult the General Protocol GP-1 “Preparation of 3-D Life Fast Gelling Hydrogels” and the 3-D Life Hydrogels User Guide on www.cellendes.com.