5x SolisFAST™ Master Mix with UNG Ready To Load

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SolisFAST® Master Mix with UNG, Ready to Load

Fast singleplex and multiplex endpoint PCR assays

For in vitro use only

Description:

SolisFAST® Master Mix with UNG Ready to Load is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up.

Applications:

– Ultra fast endpoint analysis – results in 20 minutes

– Suitable for singleplex and multiplex reactions

– High-yield PCR

– Template generation for sequencing

Benefits:

  • FAST – less time from sample to result
  • ACCURATE – specific and reliable PCR in singleplex and multiplex assays
  • STABLE – 30 day stability at room temperature
  • TRUSTWORTHY – avoid carry-over contamination with UNG mixes and get reliable results in sanger sequencing applications

Mix Composition:

SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

PCR buffer: Includes 17.5 mM MgCl2 (1X PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR reaction

Green loading dye*: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.*The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).

Shipping and Storage Conditions:

  • Routine storage at -20 °C until expiry date
  • The mix can be stored at +4 °C for up to 6 months
  • Stability at room temperature (15 – 25 °C) for 1 month
  • Freeze-thaw stability: 30 cycles

This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST® is an EU registered trademark of Solis BioDyne OÜ.

Variant
Pack/Size
Ordering
Price
Qty
5 x 1 ml
60 ml (12 x 5-pack (5 x 1 ml)/15 000 rxn) / Vial

SKU: SBD-24-22-00001-5

Manufacturer Part No: 24-22-00001-5

UNSPSC Code: 41106314

Price: £562.69 ex. VAT
1 ml
50 ml (50 x 1 ml/12 500 rxn) / Vial

SKU: SBD-24-22-00001

Manufacturer Part No: 24-22-00001

UNSPSC Code: 41106314

Price: £125.08 ex. VAT
20 ml
20 ml (5 000 rxn) / Pack

SKU: SBD-24-22-00020

Manufacturer Part No: 24-22-00020

UNSPSC Code: 41106314

Price: £1,990.56 ex. VAT

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5x SolisFAST™ Master Mix with UNG Ready To Load

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