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5x SolisFAST™ SolisGreen® qPCR Mix (no ROX)

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SolisFAST® SolisGreen® qPCR Mix (no ROX)

Fast and highly sensitive dye-based qPCR

For in vitro use only

Description:

SolisFAST® SolisGreen® qPCR Mix (no ROX) is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays. Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.

The mix contains all components necessary, except primers, nuclease-free water, and DNA template.

The mix is compatible with qPCR cyclers that do not need ROX as a passive reference signal for normalization of the data.

Applications:

– DNA/cDNA target detection

– Gene expression analysis

– Copy Number Variation (CNV) analysis

– miRNA profiling and quantification

Benefits:

  • FAST – delivers reproducible qPCR results up to 2x faster
  • ACCURATE – reliable quantification across technical replicates
  • SENSITIVE – consistent results with low- and high-copy targets
  • TRUSTWORTHY – robust performance and 30 day stability at room temperature for extra convenience

Mix Compatibility:

Bio-Rad: CFX96™, CFX384™, Opticon™, Opticon2™, MiniOpticon, Chromo4™

Qiagen: Rotor-Gene™ 3000 & 6000

Eppendorf: Mastercycler® ep realplexMastercycler®

Roche: LightCycler 480- Bio Molecular Systems: Mic

Takara: Thermal Cycler Dice (TP800)

Mix Composition:

SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

5x qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction

SolisGreen® dye: fluorescent dye detected in the FAM channel

Shipping and Storage Conditions:

  • Routine storage at -20 °C until expiry date
  • The mix can be stored at +4 °C for up to 6 months
  • Stability at room temperature (15 – 25 °C) for 1 month
  • Freeze-thaw stability: 30 cycles

This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST is a trademark of Solis Biodyne OÜ. This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST® is an EU registered trademark of Solis BioDyne OÜ. Other registered trademarks: TaqMan® and LightCycler® (Roche Diagnostics GmbH), Mastercycler® (Eppendorf AG). All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.

Variant
Pack/Size
Ordering
Price
Qty
5 x 1 ml
60 ml (12 x 5-pack (5 x 1 ml)/15 000 rxn) / Vial

SKU: SBD-28-41-00001-5

Manufacturer Part No: 28-41-00001-5

UNSPSC Code: 41106304

Price: £625.23 ex. VAT
20 ml
20 ml (5 000 rxn) / Pack

SKU: SBD-28-41-00020

Manufacturer Part No: 28-41-00020

UNSPSC Code: 41106304

Price: £2,211.82 ex. VAT
1 ml
50 ml (50 x 1 ml/12 500 rxn) / Vial

SKU: SBD-28-41-00001

Manufacturer Part No: 28-41-00001

UNSPSC Code: 41106304

Price: £138.23 ex. VAT

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5x SolisFAST™ SolisGreen® qPCR Mix (no ROX)

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