5x SolisFAST™ SolisGreen® qPCR Mix (ROX)

SolisFAST^® SolisGreen^® qPCR Mix (ROX)

Fast and highly sensitive dye-based qPCR

For in vitro use only

Description:

SolisFAST® SolisGreen® qPCR Mix (ROX) is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays. Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.

The mix contains all components necessary, except primers, nuclease-free water, and DNA template.

The mix is compatible with qPCR cyclers that need ROX as a passive reference signal for normalization of the data.

Applications:

– DNA/cDNA target detection

– Gene expression analysis

– Copy Number Variation (CNV) analysis

– miRNA profiling and quantification

Benefits:

• FAST – delivers reproducible qPCR results up to 2x faster
• ACCURATE – reliable quantification across technical replicates
• SENSITIVE – consistent results with low- and high-copy targets
• TRUSTWORTHY – robust performance and 30 day stability at room temperature for extra convenience

Mix Compatibility:

– Applied BioSystems: 7500, 7500 Fast, 7900HT Fast, QuantStudio™ 3, 5, 6, 7, 12K Flex, ViiA™ 7

Mix Composition:

– SolisFAST™ DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast oligo-based hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

– qPCR buffer: Includes 7.5 mM MgCl22 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction

– SolisGreen® dye: fluorescent dye detected in the FAM channel

Shipping and Storage Conditions:

• Routine storage at -20 °C until expiry date
• The mix can be stored at +4 °C for up to 6 months
• Stability at room temperature (15 – 25 °C) for 1 month
• Freeze-thaw stability: 30 cycles

Applied Biosystems is trademarks of Applied Biosystems LLC. QuantStudio and ViiA are trademarks of Life Technologies Corporation.