Biometra Tankblot

Key Features

Features

  • Fast, efficient and reproducible transfer of proteins
  • Effective cooling thanks to built-in cooling
  • Simultaneous blotting of up to four gels
Special Requirements?

Description

Product Info

Tank Blot Apparatus and Blotting Modules
For Gentle Blotting of Large and Temperature-Sensitive Proteins

Best suited for large proteins and native enzymes
During tank blotting proteins are transferred in a vertical buffer tank between electrodes arranged on the sidewalls onto membranes. Tank blotting is recommended particularly for blotting of large molecules (> 100 resp. 200 kDa, depending on the proteins characteristics) or of proteins which are difficult to transfer with other blotting techniques. It is the method of choice also for native and temperature-sensitive proteins. Transfer can be done fast or overnight. Thanks to the higher buffer volume transfer times of more than 24 h can be realised, e. g. for gentle blotting of very big proteins.

Different models are available: The highly sophisticated Tankblot for mini-gels offers the most effective cooling by the laterally integrated cooling jacket. Tankblot Eco-Mini and Tankblot Eco- Maxi are part of the modular Eco-Line and offer highly effective cooling of small and large gels by integrated cooling bases. The Mini-V8·10 Blot Module is designed for use in the buffer tank of the Mini-V8·10 vertical gel electrophoresis apparatus.

Tankblot
In the Tankblot a cooling jacket is integrated into the sidewalls. This results in convective chilling by circulation: the temperature is distributed more homogeneously (± 0.5 °C at 6 °C buffer temperature) than with other designs that use a magnetic stirrer circulating the buffer. Additionally this special design prevents formation of ion gradients without the need for a magnetic stirrer. Depending on the selected buffer, transfer can be fast (between 30 min. and 4 h) or, under exceptionally gentle conditions, overnight (ca. 12 h). Thanks to the buffer volume of 1,200 ml long transfer times can be realised.

Tankblot Eco-Line
Wire electrodes are placed on the side walls to provide uniform, reproducible protein transfer over a wide molecular weight range. The lower buffer chamber with built-in cooling base absorbs heat generated during rapid transfers and allows temperature controlled runs. A stir bar may be added to the buffer chamber to further improve buffer circulation and heat exchange. The colour coded blotting cassettes in combination with the colour coded connectors for the Bigfoot Safety Lid ensure accurate orientation of the gel sandwich during the transfer. The tankblot is available as stand-alone apparatus or as a module compatible with the buffer chamber EBC (with integrated cooling base) or EB (without cooling option).
The Electrophoresis Module for Eco-Mini and Eco-Maxi are available
separately and will convert the corresponding tank blotting systems
into powerful PAGE electrophoresis systems.

Tankblot Eco-Mini
Tankblot Eco-Mini is designed to transfer up to four PAGE mini-gels (up to 9.5 cm x 8.5 cm) in separate blotting cassettes simultaneously.

The special design of the blotting cassettes allows easy assembly and
loading into the blot module. Additional the assembly defends blotting sandwiches from squeezing.

Tankblot Eco-Maxi
Tankblot Eco-Maxi is the large version for gel sizes up to 22 cm x 19 cm. The system offers the capacity of multiple transfers: several small gels or 1 large gel per cassette. Up to 2 large gels can be transferred simultaneously in separate blotting cassettes.

The special design for fixing the blotting sandwich in the blotting cassette prevents squeezing and allows easy handling.

Technical Data

Multigel, Multigel-Long

Apparatus dimensions
(W x D x H)

26.5 x 16.5 x 18.7 cm (Multigel)
26.5 x 16.5 x 23.7 cm (Multigel-Long)

Gel size (W x L)

11.0 x 12.0 cm (Multigel-Long)
11.0 x 7.0 cm (Multigel)

Glass plate size (W x L)

14.8 x 9.3 cm (Multigel)
14.8 cm 14.3 cm (Multigel-Long)

Number of gels

1 or 2

Capacity

max. 24 samples per gel
(48 samples total)

Typical sample volumes

14–500 µl per well (depending on gel thickness)

Combs






Thickness
(mm)

Number of Wells

Volume per Well
(µl)

0.6

24

14

1.0

1 prep. + 2 marker lanes

2 x 40 and 5001

11

50

12

45

24

18

Buffer volume

170 ml (upper buffer chamber) + 200 ml (lower buffer chamber)

1Volume of the preparative well

Order Info

Order number

Item

Multigel Systems

846-010-200

Multigel, complete system with 2 sets of glass plates with fixed 1 mm spacers, 2 silicone rubber seals, 2 combs (24 wells) and 2 sets of clips (12 pcs)

846-010-220

Multigel, dto., but with fixed 0.6 mm spacers

846-010-230

Multigel, buffer chamber and safety lid; without accessories

846-010-300

Multigel-Long, complete system with 2 sets of glass plates with fixed 1 mm spacers, 2 silicone rubber seals, 2 combs (24 wells) and 2 sets of clips (12 pcs)

846-010-320

Multigel-Long, dto., but with fixed 0.6 mm spacers

846-010-330

Multigel-Long, buffer chamber and safety lid; without accessories

Maxigel Systems

846-010-400

Maxigel, complete system with 2 sets of glass plates with fixed 1 mm spacers, 2 silicone rubber seals, 2 combs (12 wells), 3 sets of clips (12 pcs) and 1 set of Maxi-Clips (4 pcs)

846-010-430

Maxigel, dto., but with fixed 2 mm spacers

846-010-440

Maxigel, buffer chamber and safety lid; without accessories

Order number

Multigel Accessories

846-010-201

Notched glass plate with straight edge

846-010-202

Glass plate with fixed spacers, 1.0 mm

846-010-221

Glass plate with fixed spacers, 0.6 mm

846-010-255

Silicone rubber seal, 1.0 mm (2 pcs)

846-010-256

Silicone rubber seal, 0.6 mm (2 pcs)

846-010-258

Short grey seal to be attached to the main chamber
(4 pcs)

846-010-259

Long grey seal to be attached to the main chamber
(4 pcs)

846-010-057

Clips (6 pcs)

Multigel-Long Accessories

846-010-301

Notched glass plate with straight edge

846-010-302

Glass plate with fixed spacers, 1.0 mm

846-010-321

Glass plate with fixed spacers, 0.6 mm

846-010-355

Silicone rubber seal, 1.0 mm (2 pcs)

846-010-356

Silicone rubber seal, 0.6 mm (2 pcs)

846-010-258

Short grey seal to be attached to the main chamber
(4 pcs)

846-010-359

Long grey seal to be attached to the main chamber
(4 pcs)

846-010-057

Clips (6 pcs)

Combs for Multigel and Multigel-Long

Combs, 1.0 mm thick (max. volume/well)

846-010-207

11 wells (50 µl)

846-010-203

12 wells, multichannel pipet compatible (45 µl)

846-010-204

24 wells (18 µl)

846-010-208

preparative, with 2 marker lanes (2 × 40 µl and 500 µl)

Combs, 0.6 mm thick (max. volume/well)

846-010-222

24 wells (14 µl)

Further Accessories

846-010-019

Rotary Table for twin-chambers (Minigel-Twin, Eco-Mini, Mini-V8·10, Multigel, Multigel-Long, Model V16-2)

846-010-029

Universal Glass Plate Stand with levelling feet,
4 positions for any gel size

Order number

Accessories

Maxigel Accessories

846-010-401

Notched glass plate with straight edge

846-010-402

Glass plate with fixed 1 mm spacers

846-010-403

Glass plate with fixed 2 mm spacers

846-010-057

Clips (6 pcs)

846-010-416

Maxi-clips (4 pcs)

846-010-455

Silicone rubber seal, 1.0 mm (2 pcs)

846-010-457

Silicone rubber seal, 2.0 mm (2 pcs)

846-010-459

Long grey seal to be attached to the main chamber
(4 pcs)

846-010-458

Short grey seal to be attached to the main chamber
(4 pcs)

Combs, 1.0 mm thick (max. volume/well)

846-010-404

12 wells (140 µl)

846-010-405

18 wells (90 µl)

846-010-406

24 wells (50 µl)

846-014-020

25 wells (40 µl)

846-014-022

preparative, without marker lane (1,800 µl)

846-010-407

preparative, with 2 marker lanes (2 × 50 µl and 1.600 µl)

Combs, 2.0 mm thick (max. volumen/well)

846-010-408

12 wells (280 µl)

846-010-409

18 wells (180 µl)

846-010-410

24 wells (100 µl)

846-014-021

45 wells (50 µl)

846-010-411

preparative, with 2 marker lanes
(2 × 100 µl and 3.200 µl)

Further Accessories

846-010-029

Universal Glass Plate Stand with levelling feet,
4 positions for any gel size