Description
Applications:
- Oxygen measurement in cell cultures and tissues
- Ideal for in vitro hypoxia conditions, like 3D cell cultures, spheroid models, and tissue
Oxygen monitoring is defined as the measurements of O2 in the direct neighborhood of cultured cells. Oxygen sensitive beads, with a diameter of 50 µm (CPOx), are used in combination with the ibidi OPAL system or a FLIM microscope to measure the oxygen concentration.
Technical Features:
- 50 µm polystyrene beads with an O2-sensitive fluorophore (fluorescence lifetime)
- Quantitative results with OPAL and FLIM microscopes
- Biocompatible in cell culture, spheroids,
or tissue - Not cell permeable
- For extracellular O2 monitoring
Specifications:
| Composition | 50 µm beads |
| Ex | 530 nm (510-550 nm) |
| Em | 600 nm (570-670 nm) |
| Lifetime (37°C) | 4.0 µs (21% O2) / 5.3 µs (0% O2) |
Overview: O2 Monitoring and O2 Imaging
| ibidi OPAL on Fluorescence Microscope (Phosphorescence Lifetime Measurement) |
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 |
|
CPOx Beads |
NanO2 |
| Quantitative Extracellular Not Image-Based |
Quantitative Intracellular Not Image-Based |
| PLIM Microscope (Phosphorescence Lifetime Imaging): |
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|
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| CPOx Beads |
NanO2 |
| Quantitative Extracellular Image-Based |
Quantitative Intracellular Image-Based |
| Fluorescence Microscope (Intensity-Based Imaging): |
 |
| MM2 |
| Quantitative Intracellular Image-Based |
