FIREScript RT cDNA synthesis KIT

FIREScript RT cDNA synthesis KIT

For in vitro use only

Description:

FIREScript RT cDNA Synthesis Kit contains all the reagents you need to synthesize cDNA in 7 separate vials. Its fast reaction speed allows you to perform your synthesis in just 15 minutes while the high working temperature of the enzyme (up to 60 °C) ensures high yield and great sensitivity. The kit format allows flexibility including the preincubation of complex RNA.

Applications:

– First strand cDNA synthesis

– RT-PCR

– RT-qPCR

Concentration:

200 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 °C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

– FIREScript Reverse Transcriptase

– RiboGrip RNase Inhibitor

– 10x RT Reaction Buffer with DTT

500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl₂, 100 mM DTT

– Oligo (dT) Primer

– Random Primers

– dNTP Mix

– Water, nuclease free

Shipping and Storage Conditions:

Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.