FIREScript RT cDNA synthesis MIX without primers

£190.00£615.00

Key Features

FIREScript RT cDNA Synthesis Mix contains all the reagents you need to synthesize cDNA from just 3 tubes. Its fast reaction speed allows you to perform your synthesis in just 15 minutes while the high working temperature of the enzyme (up to 60 ºC) ensures high yield and great sensitivity.

Clear
FIREScript RT cDNA synthesis MIX without primers
SKU: FIREScript RT cDNA synthesis MIX without primers Categories: ,
Special Requirements?

Description

Applications:

– First strand cDNA synthesis

– RT-PCR

– RT-qPCR

 

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

 

Reagents Provided in Product Sample:

– FIREScript Enzyme Mix

  FIREScript RT and RiboGrip RNase Inhibitor

– 10x RT Reaction Premix with Oligo (dT) and Random primers

  RT Reaction Buffer with DTT, dNTPs, Oligo (dT) primer and Random primers

– 10x RT Reaction Premix with Oligo (dT) primer

  RT Reaction Buffer with DTT, dNTPs and Oligo (dT) primer

– 10x RT Reaction Premix with Random primers

  RT Reaction Buffer with DTT, dNTPs and Random primers

– 10x RT Reaction Premix without primers

  RT Reaction Buffer with DTT and dNTPs

– Water, nuclease free  

 

Reagents Provided:

– FIREScript Enzyme Mix

  FIREScript RT and RiboGrip RNase Inhibitor

– 10x RT Reaction Premix

  RT Reaction Buffer with DTT, dNTPs and priming method of choice*

– Water, nuclease free  

 

*See product name for corresponding priming method.

 

:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.