Fuse-It-Color

£106.00£276.00

Key Features

A fusion reagent used to efficiently color-label plasma membranes of living cells

  • Fusogenic liposomes are used as a carrier for lipophilic dyes to label cellular plasma membranes
  • Superior fusion efficiency (up to 80-100%)
  • Fusion process completed within 1 – 10 minutes
  • Various emission spectra available
  • Ready to use

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Description

Applications:

  • Live cell labeling for various microscopic purposes and techniques
  • Labeling of single cell types for co-culture experiments
  • Labeling of cells for flow cytometry and FACS

After membrane fusion, cells can immediately be analyzed. Cell labeling by fusion is extremely efficient, and leads to sufficient labeling densities for most cell types already within seconds.

Specifications:

Form Solution
Concentration 3 mM
Storage -20 °C
ExMax/EmMax 484/501 nm (green)
549/565 nm (red)
644/665 nm (dark red)
750/780 nm (infrared)

From left to right: CHO cells fused with Fuse-It green, Fuse-It red, Fuse-It dred, and Fuse-It IR for 1 min.

Principle of Membrane Fusion

The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.

Additional information

Fluorescence

Green, Red, Dark Red, Infrared

Volume

100 µl, 400 µl