- Lipid imaging for monitoring turnover, traffic, and functional analysis in living cells
- Lipid raft, lipid microdomain, and lipid diffusivity measurements
- Blocking, induction, or replacement of lipid induced/regulated signal cascades
- Re-incorporation of lipids into mutant cells affected in lipid biosynthesis
After membrane fusion, cells can immediately be used for further analysis. If necessary, more than one lipid of interest can be incorporated at once. Depending on your experimental needs, the fusion process can be monitored by fluorescence microscopy.
|ExMax/EmMax||750/780 nm (infrared)|
Bodipy FL C12-galactocerebroside and TopFluor cholesterol were incorporated into the plasma membrane of CHO cells using Fuse-It-L (5 min incubation).
Principle of Membrane Fusion
The incorporation of small liposomal carriers into the plasma membrane of mammalian cells is the idea behind all of ibidi’s Fuse-It products. Liposomal carriers are able to attach and instantly fuse with plasma membranes in a physicochemical-driven manner. ibidi’s new Fuse-It reagents efficiently use this mechanism and fuse with mammalian cell surfaces immediately upon contact. Therefore, this novel technique makes the transfer of molecules independent of biological processes, such as endocytosis, pinocytosis, or specific receptor binding.