- Direct freezing at -80°C, or by using liquid nitrogen
- Tested for sterility, endotoxins, mycoplasma, fungi, and bacteria
- Serum-free – contains Bovine Serum Albumin
|Application Area||Sensitive and primary cells|
|Serum Replacement||Human Serum Albumin|
|Storage of product||4 °C|
DMSO is a supplement added to freezing media to prevent the formation of cell-harming ice crystals. However, this supplement is cytotoxic and will reduce the survival rate of sensitive cells. The ibidi Freezing Medium DMSO-Free is a unique composition that is formulated without DMSO, but still blocks the formation of ice crystals, making the product especially suitable for sensitive cell lines.
Cryopreservation of HUVEC (Human Umbilical Vein Vascular Endothelial Cells)
24 hours after thawing
48 hours after thawing
Primary cells, like endothelial cells, are very sensitive in regards to cryopreservation. This cryopreservation test, using ibidi Freezing Medium DMSO-free and HUVEC, revealed an excellent cell recovery behavior with a 97.6% average viability.