ibidi Freezing Medium DMSO-Free

Key Features

A DMSO-free, cell freezing medium especially for sensitive cells

  • Lowest cytotoxicity—compatible with sensitive cells
  • Extremely high recovery rates
  • With Bovine Serum Albumin
Special Requirements?


Technical Features

  • DMSO-free
  • Direct freezing at -80°C, or by using liquid nitrogen
  • Tested for sterility, endotoxins, mycoplasma, fungi, and bacteria
  • Serum-free – contains Bovine Serum Albumin


Application AreaSensitive and primary cells
High ThroughputNo
Serum ReplacementHuman Serum Albumin
AppearanceOptically clear
Storage of product4 °C

Why DMSO-Free?

DMSO is a supplement added to freezing media to prevent the formation of cell-harming ice crystals. However, this supplement is cytotoxic and will reduce the survival rate of sensitive cells. The ibidi Freezing Medium DMSO-Free is a unique composition that is formulated without DMSO, but still blocks the formation of ice crystals, making the product especially suitable for sensitive cell lines.

Cryopreservation of HUVEC (Human Umbilical Vein Vascular Endothelial Cells)

Before freezing

24 hours after thawing

48 hours after thawing

Primary cells, like endothelial cells, are very sensitive in regards to cryopreservation. This cryopreservation test, using ibidi Freezing Medium DMSO-free and HUVEC, revealed an excellent cell recovery behavior with a 97.6% average viability.