ibidi Gas Incubation System for CO2 and O2

Key Features

A gas mixer that upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications with CO2, O2, and humidity control

  • Ideal for live cell imaging applications – full incubator conditions on the microscope
  • Stable gas incubation without evaporation (patent pending technology)
  • Suitable for various experimental conditions (e.g., pH or hypoxia)
  • Fully compatible with the ibidi Heating Systems and stage top incubators of other suppliers
  • System includes: ibidi CO2 and O2 Gas Mixer, Humidifying Column

System includes:


ibidi Gas Mixer

Humidifying
Column

Optional:
Air Pressure
Generator
Special Requirements?

Description

Applications:

Technical Features:

  • Precise and reliable gas incubation for O2 and CO2
  • Active and fast humidification – no evaporation
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable)

The ibidi Gas Incubation System provides both humid and CO2-rich air for stage-top incubators like the ibidi Heating System. The gas mixture is continuously flushed through the stage top incubator, ensuring a maximum humidity and an optimal pH for CO2-buffered liquids.

Spheroid of breast cancer cells with living cells (green) and apoptotic center (red) due to oxygen depletion.

Components of the ibidi Gas Incubation System

ibidi Gas Mixer for CO2 and O2

  • Precise and reliable gas incubation
  • Active and fast humidification without evaporation (patent pending technology)
  • Suitable for various experimental conditions (e.g., pH or hypoxia)
  • Fully compatible with the ibidi Heating Systems
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable)

Humidifying Column

  • Humidifies the outgoing CO2 / O2 / air mix to minimize sample evaporation
  • Active humidity control inside the attached ibidi Heating System

ibidi Heating System

The ibidi Gas Incubation System upgrades your ibidi Heating System to a complete stage top incubator.

Oxygen Conditions in Various Surroundings

Location/Tissue Oxygen Partial Pressure p(O2) Corresponding O2 in Cell Culture Gas Mix (%)
Inspired air 21 kPa (=160 mm Hg) 21%
Alveolus 14 kPa (=104 mm Hg) 14%
Arterial blood 14 kPa (=104 mm Hg) 14%
Veneous blood 5 kPa (=40 mm Hg) 5%
Interstitial fluid 5 kPa (=40 mm Hg) 5%
Healthy tissue 2-3 kPa (=15- 23 mm Hg) 2-3%
Tumor without blood vessels
Outside 1 kPa (=7.5 mm Hg) 1%
Center 0 kPa (=0 mm Hg) 0%
Normoxia conditions 2 – 11 kPa (=15 – 82 mm Hg) 2-11%
Hypoxia conditions 0 – 1 kPa (=0 – 7.5 mm Hg) 0-1%
Cell culture incubator (5% CO2) 20 kPa (=150 mm Hg) 20%

Overview of the ibidi Heating & Incubation System, CO2 and O2 Control

Gas Supply (Input)

Specifications:

Gas Supply (Input)
Pressurized Air 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
CO2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
N2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)

Tubing for all gasses (Supplied with the system) PUR/PUN tubing
4 mm inner diameter
6 mm outer diameter
1 mm wall thickness

ibidi Gas Mixer

Specifications:

CO2 Control
CO2 Control Range 0.1% – 20%
Accuracy 0.1% – 0.5% (absolute)
O2 Control
O2 Control Range 0% – 21%
Accuracy 0.5 % (absolute)
Gas Flow
Control Range 5 – 20 l/h

Humidifying Column

Specifications:

Humidity Control
Control Range 20% – 99% (Rel. Humidity)
Accuracy 1% (absolute)
Water Refill Every 7 days