- Immunofluorescence assays with nuclear DAPI counterstaining
- Widefield fluorescence and confocal microscopy
- Short-term and long-term storage of stained slides and dishes
- Specially designed for mounting ibidi µ-Slides, µ-Dishes, and µ-Plates
- Contains DAPI for nuclear counterstaining, also available without DAPI
- Contains antioxidant agent (DABCO) to preserve fluorochromes
- Provides optimal pH for detecting fluorescence signals
- Non-drying, non-hardening, and volume-stable
- Low autofluorescence for reduced background
|Appearance||Liquid, optically clear|
|Refractive index nD||1.42–1.44|
|Staining specificity||DNA/RNA (nucleus)|
|DAPI-DNA (Exmax / Emmax)||358 / 461 nm|
|Storage of mounted samples||In the dark at 2–8°C|
|Product storage||In the dark at 2–8°C|
Principle of the ibidi Mounting Medium With DAPI
The aqueous, non-hardening ibidi Mounting Medium With DAPI is ideal for mounting immunofluorescence-stained samples that require counterstaining of the nucleus.
The ibidi Mounting Medium with DAPI contains a working concentration of DAPI (4′,6-diamidino-2-phenylindole), which is a convenient DNA-binding fluorescent dye. This nucleus label is a robust nuclear/chromosomal counterstain, which is the gold-standard for immunofluorescence assays.
When bound to DNA, the fluorescence intensity of DAPI is much higher than when it is in solution. This enables direct fluorescence microscopy and the long-term storage of slides mounted with the ibidi Mounting Medium with DAPI.
With an excitation maximum of 358 nm and an emission maximum of 461 nm, the DAPI fluorescence signal exhibits a minimal spectral overlap with other frequently used fluorescence dyes in immunocytochemistry (e.g., GFP and similar dyes with even longer wavelengths).
The dropper bottle of the ibidi Mounting Medium With DAPI is suitable for convenient and easy filling of all sorts of slide and dish types (e.g., ibidi µ-Dishes, Chambered Coverslips, and Channel Slides).
Low Bleaching of Fluorochromes in Stained Samples
The ibidi Mounting Medium With DAPI contains the anti-bleaching reagent 1,4–diazabicyclo[2.2.2]octane (DABCO). This antifade agent drastically reduces photobleaching of fluorochromes in immunofluorescence microscopy. This preserves a strong fluorescence signal in the sample.
High Signal and Low Background Fluorescence
The ibidi Mounting Medium With DAPI gives the best signal-to-noise-ratio on the market. Competitor products show a lower DAPI signal and a higher background fluorescence.