Fully Automated Nucleic Acid Extraction
In addition to automated protocols for bacteria, viruses, as well as human, animal and plant tissues, customers can easily process complex forensic samples and highly processed food. The system also provides enrichment routines for cell-free nucleic acids and allows users to conduct bisulfite conversion for epigenetic analyses. High-quality magnetic and paramagnetic particles act as the solid phase for binding nucleic acids. Buffer conditions are set both to create a fine particle dispersion in solution and to ensure fast, quantitative sedimentation during collection, while the multifunctional heating/magnetic unit (IHMU – Intelligent Heating/Magnet Unit) is moved into position under the bottom of the reagent cartridges. The liquid can then be reliably aspirated without transferring magnetic particles. The heating function supports the automated process during lysis, drying and elution. Residual solvent, which can potentially inhibit subsequent applications, are removed effectively, and nucleic acids are efficiently desorbed in the elution buffer.
Rounding out the overall application is the use of patented
DC-Technology® (Dual-Chemistry). The use of variable buffer systems while simultaneously reducing the concentrations and/or ionic strengths required creates the perfect conditions for proteolytic lysis and reduces the potential risk of transferring salts into the eluted sample. This results in optimized workflow times and the extraction of high-quality nucleic acids.
Intelligent kit architecture
When used with the InnuPure® C16 touch, ready-to-use extraction kits greatly simplify routine laboratory work while saving resources. The timeconsuming job of filling reservoirs, for example, is a thing of the past. Prefilled cartridges also eliminate the risks of filling the wrong tubes and of spilling reagent. Most importantly, however, having so many kit elements prepared in advance saves valuable time. All kits come in two types of packaging: one that includes Reagent Strips for individual extraction processes for small numbers of samples and one with reagent plates for up to 16 extractions at medium sample volumes.This limits prep work for the InnuPure® C16 touch to simply loading the sample tray.
Core Liquid Handling Competencies
The use of a highly precise pipetting system makes InnuPure® C16 touch workflows accurate and reproducible. In addition, the high-quality pipette tips used guarantee optimal while minimizing the amount of liquid remaining in the tips. The integrated dispensing unit allows operators to set the elution volume to a value between 20 and 500 μl; transfer is then automatic. The pipette tips and Elution Tubes are all included as components of the extraction kit.