NanO2 Nanoparticles for Intracellular O2 Imaging

£319.00

Key Features

Nanoparticle reagents for the quantitative imaging of intracellular O2 concentration (to be used with FLIM or Fluorescence Microscopes)

  • Exact oxygen imaging inside cells
  • Fast measurements within seconds
  • Quantitative results
  • 100 µg (10–100 assays)

in cooperation with

logo_luxcel.jpg

SKU
74151
£319.00
Special Requirements?

Description

Applications:

  • Monitoring in situ oxygenation of cell culture
  • Measuring the 3D distribution of oxygen in spheroids or tissue
  • Studying metabolic processes in cancer research studies
  • Investigating infections in cell cultures, like bacteria and fungi

For intracellular oxygen imaging, an oxygen-sensitive nanoparticle reagent is brought into the cells. FLIM microscopes image quantitative O2 concentrations by identifying a change in the lifetime of the fluorophore NanO2. With a standard fluorescence (or a confocal) microscope, a quantitative O2 concentration can also be determined by measuring the fluorescence intensity of the ratiometric fluorophore reagent MM2.

Technical Features:

  • O2-sensitive fluorophore (fluorescence lifetime)
  • Quantitative results with OPAL and FLIM microscopes
  • Biocompatible in cell culture, spheroids, or tissue
  • Directly cell permeable, self-loading
  • For intracellular image-based O2 measurement

Specifications:

Composition Nanoparticle reagent
Ex 400 nm (390-405 nm)
Em 650 nm (640-670 nm)
Lifetime (37°C) 23 µs (21% O2)
65 µs (0% O2)

Principle of FLIM – Fluorescence Lifetime Imaging

Some fluorophores stay in their excited state for a relatively long time. This time period is called the fluorescence lifetime. FLIM microscopes and OPAL measure this lifetime, instead of measuring the intensity.

Some long-lifetime fluorophores show a strong dependency on the surrounding oxygen level. By measuring the fluorescence lifetime, you are able to optically quantify the oxygen concentration.

In the example on the right, a 3D cell spheroid is measured optically for oxygen concentration. All cells were labeled with NanO2 and imaged with a widefield FLIM microscope. O2 concentration can be easily calculated from the fluorescence lifetime. The higher the oxygen concentration is, the shorter the fluorescence lifetime.

Overview: O2 Monitoring and O2 Imaging

ibidi OPAL
on Fluorescence Microscope

(Phosphorescence Lifetime Measurement)
CPOx Beads
NanO2
Quantitative
Extracellular
Not Image-Based
Quantitative
Intracellular
Not Image-Based
PLIM Microscope
(Phosphorescence Lifetime Imaging):
CPOx Beads
NanO2
Quantitative
Extracellular
Image-Based
Quantitative
Intracellular
Image-Based
Fluorescence Microscope
(Intensity-Based Imaging):
MM2
Quantitative
Intracellular
Image-Based