- Fluorescence microscopy, live cell imaging, and confocal fluorescence microscopy
- Imaging of fluorescent proteins after transient or stable plasmid DNA transfection
- Protein localization with fluorescence labels
Lipid based transfection reagents often interact with plastic surfaces and therefore affect transfection efficiency and cell viability. ibidi’s Torpedo DNA reagent has been specifically optimized for use with ibidi’s plastic materials. This results in a reagent with very low cytotoxicity as well as high transfection efficiency.
|Composition||Proprietary cationic lipids|
|Assays||Up to 500 (24-well) or up to 150 (6-well)
with 0.5 ml reagent
HT-1080 Cells Show High Transfection Efficiency After Transfection with Torpedo DNA
HT-1080 cells were transfected with pCMV LifeAct-TagGFP2 using Torpedo DNA. The cells were imaged 24 hours afterwards showing high transfection efficiency.
Transfection Protocol for µ-Slide 8 Well: