WiScan® Hermes 24/7 Fully Autonomous Imaging System

Key Features

  • Incubates plates in a built-in plate stacker which accommodates up to 20 plates under incubation conditions (37ºC, 5% CO2)
  • Loads & unloads plates for imaging
    • All external sources supported –Incubators, stackers, liquid handlers
  • Acquires high quality images on the WiScan® Hermes automated high content screening system
  • Produces presentation-ready results using multiple visualization tools (scatter plots, histograms, heat maps, time graphs, dose curves, etc.)
Special Requirements?

Description

The customer-inspired Hermes 24/7 establishes a new standard for simplicity and compactness in completely automated, image-based experimentation.

IDEA Bio-Medical’s in-house expertise in areas of precision machine design, manufacture and software development enabled the creation of the Hermes 24/7 fully automated sample-manipulator and high content imaging system.

In designing the new Hermes 24/7 automated system, they aimed to empower labs to utilize existing infrastructure to expand their capabilities while minimizing the amount of valuable lab space occupied.

With push-button programming the system autonomously:

  • Incubates plates in a built-in plate stacker which accommodates up to 20 plates under incubation conditions (37ºC, 5% CO2)
  • Loads & unloads plates for imaging
    All external sources supported –Incubators, stackers, liquid handlers
  • Acquires high quality images on the WiScan® Hermes automated high content screening system
  • Analyzes acquired images using WiSoft® Athena automated image analysis software
  • Produces presentation-ready results using multiple visualization tools (scatter plots, histograms, heat maps, time graphs, dose curves, etc.)

Specifications:

Features Content/Description
3D reader EPI-fluorescence inverted optics mounted on XYZ (patented) linear scanner
Auto Focus Patented ultra-fast laser-based Auto Focus with 100nm resolution
XY motion Accurate positioning with 200nm repeatability
Illumination sources 7 optional LED sources (DAPI,CFP,GFP,YFP,RFP,mCherry,CY5). Transmission: White LED source
Optical Filters 2 emission filters and compatible dichroic filters (automatically exchanged)
Objectives (Air) Choice of air objectives in the range: 2X to 60X, high NA.
Camera High sensitivity CCD camera with 1.3MPixel resolution
Sample format Supports full-area screening of 6-1536 well plates Supports slides, microarrays, 35 mm dish formats. U-shaped bottom plats are optional.
Computer PC with Windows® operating system and touch screen.
Enclosure Allows operation in fully lit areas
Desktop standalone platform 47 W x 72 D x 57 H (cm), 18.5 W x 28.5 D x 22.4 H (inches) With plate cover closed
Certification CE, UL

Live Cell Imaging Module:

Features Content/Description
Live cell imaging Allow long time lapse experiments. Sample does not move during most of the scanning process.
Live cell conditions
  • Temperature control. Range – from ambient+5°C to 40°C ±0.5°C
  • CO2 level control– enables setting of 0.02 L/min- 0.13 L/min mixed with air for desired CO2 percentage. (external accessory). CO2 Range: 0-15%. Set Point Resolution: 1%
  • Humidity

Object Mapping (Rare Event Detection) Module:

Features Content/Description
Object mapping for rare events detection
  • First scan: HTS with low magnification, for efficient region of interest object mapping.
  • Second scan: HCS with high magnification of detected objects
Analysis tools WiSoft® based image processing tools for object definition and detection in the first low magnification scan and further tools to analyze the detected objects with high magnification
Objectives Automatic objective exchanger of up to 3 objectives at a time
Throughput Enhances the throughput of high magnification scanning of rare objects
Statistical tools Provides automatic decision resulting in efficient screening for minimum required objects in a well

High Throughput (enhanced acquisition speed) Module:

Features Content/Description
Combination of HCS and HTS Ultra-fast High Content Screening unit
Image acquisition speed >10 images per second (depends on experiment conditions)
Throughput A full 96-well plate screening using 10X magnification with a single field per well, three color fluorescence, 150 ms total exposure time per field, runs in ~2 minutes. With some applications, this will include data processing and analysis results simultaneously with image acquisition.

Automation Module (interfacing to external robotic arms):

Features Content/Description
Remote accessibility Protocols ( applications and screening parameters ) execution and management
External loader compatibility (Robot/manipulator)
  • Communication channel for control by external equipment
  • Loading door with automatic opening/closing synchronized with external equipment
External loader(Robot/manipulator) integration Full integration support
Spheroids Multiplexed scan Thymocites Neurons Mitochondria+Nuclei
Macrophages expressing TNF-a RNA (red) in response to zymosan (green). Intranuclear Foci Fibroblasts 40×0.75NA 4 colour Cytoskeleton Uptake of fluorescent zymosan (green) by human monocyte-derived macrophages (blue nuclei).