WiScan® HERMES Automated High Content Imaging System

Key Features

  • Phenotypic Screening
  • Spheroids and 3D Models
  • Rare-event detection
  • Cytometry
  • Cell Counting
  • Protein Expression
  • Cell Morphology changes
  • Cell cycle tracking
Special Requirements?

Description

Easily generate publication-quality images at high throughput speeds

WiScan® Hermes is a cost-effective system that is both sophisticated and flexible, offering up to 7 fluorescence colours, bright field option, and a large range of air objectives. The system can accommodate a variety of multi-well plates and sample formats (slides, dishes…) and offers environmental control for live cell assays.

Versatile

Whether you are performing assay development, compound screens, transfection assays or looking at a few samples in great detail, and whether you are using 3D models (such as spheroids, Organoids), Zebrafish imaging, primary cells, fixed cells or live cell imaging, WiScan® Hermes is the solution for you.

Reliable

WiScan Hermes, IDEA Bio-Medical’s automated imaging system for high content screening (HCS) provides the unique combination of the two contradicting primary functions of automated microscopy: Image quality and acquisition speed.

Robust

WiScan® Hermes’ mechanisms are based on patents, creatively designed to meet heavy duty operation demands (24/7) with full process robustness.

Flexible

WiScan® Hermes is a cost-effective system that is both sophisticated and flexible, offering 7 fluorescence colors, bright field option, and a large range of air objectives. The system can accommodate a variety of multi-well plates and sample formats (slides, dishes…) and offers environmental control for live cell assays.

Intuitive

It doesn’t matter if you are a beginner or an experienced microscopist, Hermes easily allows you to look deeper into your samples. The system is intuitively operated. Its built-in applications are extremely easy to use, and are operated at the push-of-a-button.

Specifications:

FeaturesContent/Description
3D readerEPI-fluorescence inverted optics mounted on XYZ (patented) linear scanner
Auto FocusPatented ultra-fast laser-based Auto Focus with 100nm resolution
XY motionAccurate positioning with 200nm repeatability
Illumination sources7 optional LED sources (DAPI,CFP,GFP,YFP,RFP,mCherry,CY5). Transmission: White LED source
Optical Filters2 emission filters and compatible dichroic filters (automatically exchanged)
Objectives (Air)Choice of air objectives in the range: 2X to 60X, high NA.
CameraHigh sensitivity CCD camera with 1.3MPixel resolution
Sample formatSupports full-area screening of 6-1536 well plates Supports slides, microarrays, 35 mm dish formats. U-shaped bottom plats are optional.
ComputerPC with Windows® operating system and touch screen.
EnclosureAllows operation in fully lit areas
Desktop standalone platform47 W x 72 D x 57 H (cm), 18.5 W x 28.5 D x 22.4 H (inches) With plate cover closed
CertificationCE, UL

Live Cell Imaging Module:

FeaturesContent/Description
Live cell imagingAllow long time lapse experiments. Sample does not move during most of the scanning process.
Live cell conditions
  • Temperature control. Range – from ambient+5°C to 40°C ±0.5°C
  • CO2 level control– enables setting of 0.02 L/min- 0.13 L/min mixed with air for desired CO2 percentage. (external accessory). CO2 Range: 0-15%. Set Point Resolution: 1%
  • Humidity

Object Mapping (Rare Event Detection) Module:

FeaturesContent/Description
Object mapping for rare events detection
  • First scan: HTS with low magnification, for efficient region of interest object mapping.
  • Second scan: HCS with high magnification of detected objects
Analysis toolsWiSoft® based image processing tools for object definition and detection in the first low magnification scan and further tools to analyze the detected objects with high magnification
ObjectivesAutomatic objective exchanger of up to 3 objectives at a time
ThroughputEnhances the throughput of high magnification scanning of rare objects
Statistical toolsProvides automatic decision resulting in efficient screening for minimum required objects in a well

High Throughput (enhanced acquisition speed) Module:

FeaturesContent/Description
Combination of HCS and HTSUltra-fast High Content Screening unit
Image acquisition speed>10 images per second (depends on experiment conditions)
ThroughputA full 96-well plate screening using 10X magnification with a single field per well, three color fluorescence, 150 ms total exposure time per field, runs in ~2 minutes. With some applications, this will include data processing and analysis results simultaneously with image acquisition.

Automation Module (interfacing to external robotic arms):

FeaturesContent/Description
Remote accessibilityProtocols ( applications and screening parameters ) execution and management
External loader compatibility (Robot/manipulator)
  • Communication channel for control by external equipment
  • Loading door with automatic opening/closing synchronized with external equipment
External loader(Robot/manipulator) integrationFull integration support
SpheroidsMultiplexed scanThymocitesNeuronsMitochondria+Nuclei
Macrophages expressing TNF-a RNA (red) in response to zymosan (green).Intranuclear FociFibroblasts 40×0.75NA 4 colourCytoskeletonUptake of fluorescent zymosan (green) by human monocyte-derived macrophages (blue nuclei).

Configuration Options

Researchers /Academia product line:

Live Cell HT: Hermes Real Time Observer ProLive Cell Basic: Hermes Real Time ObserverEntry Level: Hermes Lab Partner
Fluorescence channels442
Transmitted light (Bright Field)
Laser auto focus
Live cell experiments
Z stack for 3D imaging
Object Mapping (rare event detection)
Objectives Included3 objectives selectable from 2X-60X air objectives with high NA
WiSoft® Minerva developers analysis SW
Enhanced Throughput speed

Pharma industry product line:

Live Cell HT 7clrs: Hermes Supreme VisionIndustrial HT 7clrs: Hermes Drug Discoverer ProIndustrial HT: Hermes Drug Discoverer
Fluorescence channels774
Transmitted light (Bright Field)
Live cell experiments
Laser auto focus
Z stack for 3D imaging
Object Mapping (rare event detection)
Objectives Included3 objectives selectable from 2X-60X air objectives with high NA
WiSoft® Minerva developers analysis SW
Enhanced Throughput speed
Automation interface to robotic arms

Publications

TLR4-Mediated Pathway Triggers Interferon-Independent G0 Arrest and Antiviral SAMHD1 Activity in Macrophages

Petra Mlcochova, HelenaWinstone, LorenaZuliani-Alvarez, Ravindra K.Gupta, Cell ReportsVolume 30, Issue 12, 24 March 2020, P 3972-3980.e5

WEB LINK

The multi-kinase inhibitor lenvatinib interacts with the HDAC inhibitor entinostat to kill liver cancer cells

Jane L. Roberts, Andrew Poklepovic, Laurence Booth, Paul Dent, Cellular Signalling, 2020, 109573, ISSN 0898-6568

WEB LINK

Fingolimod Augments Monomethylfumarate Killing of GBM Cells

Dent Paul, Booth Laurence, Roberts Jane L., Poklepovic Andrew, Hancock John F.,Frontiers in Oncology  vol.10, 2020

WEB LINK 

(Curcumin+sildenafil) enhances the efficacy of 5FU and anti‐PD1 therapies in vivo.

Dent, P, Booth, L, Roberts, JL, Poklepovic, A, Hancock, JF.,  J Cell Physiol2020; 1– 13. https://doi.org/10.1002/jcp.29580 

WEB LINK

Golgi organization is regulated by proteasomal degradation.

Eisenberg-Lerner, A., Benyair, R., Hizkiahou, N. et al., Nature Communications 11, 409 (2020). https://doi.org/10.1038/s41467-019-14038-9 

WEB LINK

GZ17‐6.02 initiates DNA damage causing autophagosome‐dependent HDAC degradation resulting in enhanced anti‐PD1 checkpoint inhibitory

Booth, L, Roberts, JL, West, C, Von Hoff, D, Dent, P., J Cell Physiol.,  2020; 1– 16. https://doi.org/10.1002/jcp.29464

WEB LINK

Evasion of a human cytomegalovirus entry inhibitor with potent cysteine reactivity is concomitant with the utilisation of a heparan sulfate proteoglycan independent route of entry

M.J. Murray, N.I. BonillaMedrano, Q.L. Lee, S.J. Oxenford, R. Angell, D.P. Depledge, M.B. Reeves, Journal of Virology ,Jan 2020, JVI.02012-19; DOI: 10.1128/JVI.02012-19

WEB LINK

Enhanced signaling via ERBB3/PI3K plays a compensatory survival role in pancreatic tumor cells exposed to [neratinib+ valproate]‏

P Dent, L Booth, A Poklepovic, D Von Hoff, JF Hancock, Cellular Signalling, Jan 2020‏

WEB LINK

Seronegative patients vaccinated with cytomegalovirus gB-MF59 vaccine have evidence of neutralising antibody responses against gB early post-transplantation

I. Baraniak et al., EBioMedicine, Nov 2019

WEB LINK

Mechanisms of extracellular vesicle uptake in stressed retinal pigment epithelial cell monolayers

C. Nicholson et al., Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease, Nov 15 2019

WEB LINK

Dissecting the initial events of human cytomegalovirus cell entry

Matthew James Murray 2019

WEB LINK

Regorafenib Enhances Lethality of Sildenafil and Curcumin in Colorectal Cancer Cells

KB Owusu, M Director, P Dent , VCU THESES AND DISSERTATIONS, Feb 2019‏

WEB LINK

Differences in the Emission of Volatile Organic Compounds (VOCs) between Non-Differentiating and Adipogenically Differentiating Mesenchymal Stromal/Stem Cells from Human Adipose Tissue

Ann-Christin Klemenz et al., Cells 2019, 8(7), 697, 10 July 2019

WEB LINK

Cooperativity between stromal cytokines drives the invasive migration of human breast cancer cells

Y Elisha et al., The Royal Society Volume 374, Issue 1779, 01 July 2019

WEB LINK

Neratinib inhibits Hippo/YAP signaling, reduces mutant K-RAS expression, and kills pancreatic and blood cancer cells

P Dent et al., Oncogene (2019)

WEB LINK

IFN-independent G0 arrest and SAMHD1 activation following TLR4 activation in macrophages

P. Mlcochova, H. Winstone, L. Zuliani-Alvarez, R.K. Gupta, bioRxiv 741124

WEB LINK

Palbociclib augments Neratinib killing of tumor cells that is further enhanced by HDAC inhibition

L Booth et al., Cancer Biology &Therapy, 5 September 2018

WEB LINK

The CHK1 inhibitor SRA737 synergizes with PARP1 inhibitors to kill carcinoma cells

Laurence Booth, Jane Roberts, Andrew Poklepovic & Paul Dent, Cancer Biology & Therapy, 19 July 2018

WEB LINK

Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1

Adutler-Lieber et al., Front. Immunol., 11 June 2018

WEB LINK

Valproate augments Niraparib killing of tumor cells

Laurence Booth et al., Cancer biol ther., June 2018

WEB LINK

Protection from  cytomegalovirus viremia following glycoprotein B vaccination is not dependent on neutralizing antibodies

Baraniak et al.,PNAS April 23, 2018 

WEB LINK

The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair

Baranes-Bachar et al., Molecular Cell, Volume 69, Issue 5, 1 March 2018, Pages 866-878.e7

WEB LINK

TP53 is required for BECN1-and ATG5-dependent cell death induced by sphingosine kinase 1 inhibition‏

Santiago Lima et al., Autophagy, Jan 25, 2018

WEB LINK

[Neratinib + Valproate] exposure permanently reduces ERBB1 and RAS expression in 4T1 mammary tumors and enhances M1 macrophage infiltration.

Booth et al., Oncotarget, 26;9(5):6062-6074Dec 2017  

WEB LINK

The levels of mutant K-RAS and mutant N-RAS are rapidly reduced in a Beclin1 / ATG5 -dependent fashion by the irreversible ERBB1/2/4 inhibitor neratinib

Laurence Booth t al., Cancer biology & therapy, volume 19, issue 2, 2018

WEB LINK

Curcumin interacts with sildenafil to kill GI tumor cells via endoplasmic reticulum stress and reactive oxygen/ nitrogen species

Jane L. Roberts, Andrew Poklepovic and Laurence Booth, Oncotarget, August 2017

WEB LINK

DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV‐1 infection of macrophages

Petra Mlcochova,* , Sarah J Caswell, Ian A Taylor, Greg J Towers & Ravindra K Gupta, The EMBO Journal, October 2017

WEB LINK

Substrate-bound CCL21 and ICAM1 combined with soluble IL-6 collectively augment the expansion of antigen-specific murine CD4+ T cells

Shimrit Adutler-Lieber, et al., Blood Advances Journal, Volume 1, Issue 15, pp 1016-1030, May 13, 2017

WEB LINK

A G1‐like state allows HIV‐1 to bypass SAMHD1 restriction in macrophages

Petra Mlcochova et al., The EMBO Journal, Volume 36, Issue 2 , pp 129 – 244, January 25, 2017

WEB LINK

PDE5 inhibitors enhance the lethality of pemetrexed through inhibition of multiple chaperone proteins and via the actions of cyclic GMP and nitric oxide

Laurence Booth, Jane L. Roberts, Andrew Poklepovic, Sarah Gordon, Paul Dent, Impactjournals- Oncotarget, Advance Publications 2016, November 2016, changes 5: 9

WEB LINK

Lipopolysaccharide induces proliferation and osteogenic differentiation of adipose-derived mesenchymal stromal cells in vitro via TLR4 activation

Nicole Herzmann, Achim Salamon, Tomas Fiedler and Kirsten Peters, Experimental Cell Research, https://dx.doi.org/10.1016/j.yexcr.2016.11.012

WEB LINK

The commonly used nonionic surfactant Span 80 has RXRα transactivation activity, which likely increases the obesogenic potential of oil dispersants and food emulsifiers

Bowers RR , Temkin AM , Guillette LJ , Baatz JE , Spyropoulos DD; Gen Comp Endocrinol., 2016 Apr . doi:10.1016/j.ygcen.2016.04.029. PMID: 27131391.

WEB LINK

Rationally repurposing Ruxolitinib (Jakafi®) as a solid tumor therapeutic     

Tavallai Mehrad, Booth Laurence, Roberts Jane L, Poklepovic Andrew, Dent Paul ; Frontiers in Oncology ,vol.6,2016      

WEB LINK

Effects of crude oil/dispersant mixture and dispersant components on PPARγ activity in vitro and in vivo: identification of dioctyl sodium sulfosuccinate (DOSS; CAS #577-11-7) as a probable obesogen.

Temkin AM et al.; Environ Health Perspect, 2016, 124:112–119

WEB LINK

 Nexavar/Stivarga and ViagraInteract to Kill Tumor Cells

Tavalla et al., Journal of Cellular Physiology, 2015, 230: 2281–2298

WEB LINK

PDE5 Inhibitors Enhance Celecoxib Killing in Multiple Tumor Types

Booth L. et al., Journal of Cellular Physiology, May 2015, Volume 230, Issue 5, pages 1115–1127,

WEB LINK

Registered report: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment

Evans J. et al., August 2015, eLife 2015;4:e07301. DOI: 10.7554/eLife.07301

WEB LINK

GRP78 / BiP / HSPA5 / Dna K is a universal therapeutic target for human disease

Booth L. et al.; Journal of Cellular Physiology, 2015, 230(7): 1661-76

WEB LINK

Differential regulation of autophagy and cell viability by ceramide species

Nichola Cruickshanks , Jane L Roberts , M Danielle Bareford , Mehrad Tavallai , Andrew Poklepovic , Laurence Booth , Sarah Spiegel , Paul Dent ,Cancer Biology & Therapy  Vol. 16, Iss. 5, 2015

WEB LINK

Variomics screen identifies the reentrant loop of the calcium-activated chloride channel ANO1 that facilitates channel activation

Bill A. et al.;  Journal of Biological Chemistry, 2015, 290: 889-903

WEB LINK

High-throughput fluorescence imaging approaches for drug discovery using in vitro and in vivo three-dimensional models

Natalia J Martinez , Steven A Titus , Amanda K Wagner , Anton Simeonov  ,Expert Opinion on Drug Discovery  Vol. 10, Iss. 12, 2015

WEB LINK

Celecoxib enhances [sorafenib + sildenafil] lethality in cancer cells and reverts platinum chemotherapy resistance

Timothy Webb , Jori Carter , Jane L Roberts , Andrew Poklepovic , William P McGuire , Laurence Booth , Paul Dent , Cancer Biology & Therapy  Vol. 16, Iss. 11, 2015

WEB LINK

3,5-Diamino-1,3,4-triazoles as a novel scaffold for potent, reversible LSD1 (KDM1A) inhibitors

Kutz CJ. Et al.; Medicinal Chemistry Communications, 2014, 5, 1863-1870

WEB LINK

Regulation of OSU-03012 Toxicity by ER Stress Proteins and ER Stress–Inducing Drugs

Booth L. et al.; Mol Cancer Ther, October 2014, 13; 2384

WEB LINK

The Cofilin Phosphatase Slingshot Homolog 1 (SSH1) Links NOD1 Signaling to Actin Remodeling

Bielig H. et al.; PLOS Pathogens, 2014, 10, 9.

WEB LINK

Soft/elastic nanopatterned biointerfaces in the service of cell biology

Platzman l. et al.; METHODS IN CELL BIOLOGY; 2014, 119:237-260

WEB LINK 

HIV-1 evades innate immune recognition through specific cofactor recruitment.

Rasaiyaah J. et al.; Nature, 2013 Nov 21;503(7476):402-5

WEB LINK

Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor

Shahal T. et al.; Biointerphases  ,2012, Volume 7, Issue 1

WEB LINK