µ-Slide 18 Well Glass Bottom


Key Features

  • Pack of 15
  • All-in-one 18 well chamber slide for cost-effective experiments with small cell numbers and low reagent volumes
  • Ideal for high-resolution imaging through the No. 1.5H glass bottom with the highest optical quality and low thickness variability
  • Suitable for most microscopy techniques (e.g., TIRF, super-resolution microscopy, DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, and LSFM)

Special Requirements?


A chambered coverslip with 18 wells for cell culture, immunofluorescence, and high-end microscopy.

The µ-Slide 18 Well is a versatile live cell imaging chamber slide, suitable for a range of imaging applications. The split chamber design allows for cost effective experiments with small cell numbers and low reagent volume. Combined with a 1.5H glass bottom, this slide is suitable for both TIRF and high resolution fluorescence microscopy.

Applications Include:

  • Cultivation and high-resolution microscopy of cells
  • TIRF and single molecule applications of living and fixed cells
  • Super-resolution microscopy
  • Immunofluorescence staining and fluorescence microscopy of living and fixed cells
  • Live cell imaging over extended time periods
  • Transfection assays
  • Differential interference contrast (DIC) microscopy when used with a DIC lid

Technical Features

  • Chambered glass coverslip with 18 independent wells
  • Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
  • May require coating to promote cell attachment
  • Imaging chamber slide with excellent optical quality for high-end microscopy
  • Closely fitting lid for low evaporation
  • Individual well walls for minimizing well-to-well crosstalk and contaminations
  • Compatible with staining and fixation solutions
  • Also available with an ibidi Polymer Coverslip Bottom for superior cell growth: µ-Slide 18 Well
  • Also available as an adhesive version without a bottom: sticky-Slide 18 Well
Outer dimensions (w x l)25.5 x 75.5 mm²
Number of wells18
Dimensions of wells (w x l x h)5.7 x 6.1 x 6.8 mm³
Volume per well100 µl
Height with/without lid8.2/6.8 mm
Growth area per well0.34 cm²
Coating area per well1.15 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm


Instructions u-Slide 18 Well – Glass Bottom

Application Notes

AN 08 – Cell Culture Coating
AN 58 Immonofluorescence with u-Slide 18 Well

Application Guides

2020 Immunofluorescence
μ-Slide 18 well