- Immunofluorescence assays using standard coverslip techniques
- Long-term sample storage
- 3 fold sample preparation of adherent cells and tissue samples
- Self-adhesive silicone gasket mounted on a standard glass slide
- Biocompatible silicone material
- Non-fluorescent glass slide with frosted ends for easy handling and labeling
- Suitable for use with upright and inverted microscopes, after staining and mounting with a coverslip
- Staining volume reduction by glass coverslip
- Compatible with all common mounting media
- Optional: The gasket may be transferred to any flat clean surface for cell cultivation
|Number of wells||3|
|Dimensions of wells (w x l x h) in mm||16.5 x 16.5 x 8.0|
|Volume per well||1100 µl (150 µl*)|
|Growth area per well||1.66 cm²|
|Coating area per well||3.73 cm²|
|Suitable coverslip size||24 mm x 60 mm|
|Bottom||Glass slide (26 x 76 x 1 mm)|
* Volume reduction for staining when using a 15 mm glass coverslip (Cat. No. 10815)
Standard Procedure 3 Well | 8 Well | 12 Well Chamber, removable:
Low Volume Staining Procedure 3 Well Chamber, removable:
Which mounting medium is recommended for mounting glass slides and coverslips in the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable?
Can the ibidi Mounting Medium be used with the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable?
Is the silicon gasket of the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable reusable?
Can I use the 3 Well Chamber, removable without the 15 mm glass coverslip?
Petra Weber, Aalen University, Optical Engineering, Aalen, Germany
“We have tested the Removable Chambers (3 Well and 8 Well) and were very happy. Due to our very sensitive TIRF microscopy with an upright microscope we need appropriate glass slides with removable chambers. We tested adherent HeLa cells to detect Förster Resonance Energy Transfer between EGFR-CFP and Grb2-YFP with Fluorescence Lifetime Imaging. In particular for the activation or inhibition of intracellular signaling pathways, we need the subdivisions on the object slide. It was very easy to remove the chambers. Thank you very much for the possibility to test the chambers for free. I am sure I will use them again.”
Aalen University, Optical Engineering