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5x HOT FIREPol® Probe qPCR Mix Plus (ROX)

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5x HOT FIREPol® Probe qPCR Mix Plus (ROX)

For in vitro use only

Description:

HOT FIREPol®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

Mix Compatibility:

Appilied Biosystems: QuantStudioTM 12K Flex, ViiATM7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM

Stratagene: MX3000PTM , MX3005PTM

Mix Composition:

– HOT FIREPol® DNA Polymerase

– 5x Probe qPCR buffer

– 15 mM MgCl2 1x PCR solution – 3 mM

– dNTPs

– ROX dye

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (ROX).

Applied BioSystems and ROX are trademarks of Applied Biosystems LLC, ViiA and QuantStudio are trademark of Life Technologies Corporation; Stratagene, MX3000P and MX3005P are trademarks of Stratagene Corporation.

Variant
Pack/Size
Ordering
Price
Qty
7096_Probe_ROX_web
5× HOT FIREPol® Probe qPCR Mix Plus (ROX) - 20 ml (5000 rxn)
20 ml (5000 rxn) / Pack

SKU: SBD-08-14-00020

Manufacturer Part No: 08-14-00020

UNSPSC Code: 41106304

Price: £1,513.62 ex. VAT
7096_Probe_ROX_web
5× HOT FIREPol® Probe qPCR Mix Plus (ROX) - 1 ml (250 rxn)
1 ml (250 rxn) / Pack

SKU: SBD-08-14-00001

Manufacturer Part No: 08-14-00001

UNSPSC Code: 41106304

Price: £85.58 ex. VAT

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5x HOT FIREPol® Probe qPCR Mix Plus (ROX)

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