Description
HOT FIREPol® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. Proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets.
HOT FIREPol® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5' flap endonuclease activity.
The Mix contains internal passive reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiring platforms.
The Mix contains dUTPs to prevent cross-contamination with UNG treatment.
Applications
- DNA/LNA hydrolysis probe-based assays
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
