Solis BioDyne Oö

SOLIScript® 1-step Multiplex Probe Kit (Purple)

  • cDNA synthesis up to 60°C for superior specificity
  • analyze 1-4 targets in 1 reaction
  • reaction set-up and shipment without dry ice
  • robust amplification of GC-rich targets
  • contains dUTP to prevent cross-contamination when used in combination with UNG
  • RNase inhibitor included in kit
  • Find out more from the video HERE
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    £360.83 ex. VAT VAT
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    Description

    SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.

    Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.

    Applications

    • Detection and quantification of RNA targets with qPCR
    • DNA/LNA hydrolysis probe based assays
    • Suitable for singleplex and multiplex reactions

    Properties

    Sample type: RNA

    Product format: Reagent kit 

    Detection type: Probe-based

    Reference dye: Purple

    Compatible real-time instruments: 7500 Fast Real-Time PCR System, 7500 Real-Time PCR System, ViiA™ 7 PCR System, and QuantStudio™ 6 Flex, 7 Flex, and 12K Flex Real-Time PCR systems. Check Your cycler!

    Reagents 

    5x One-step Multiplex Probe Mix (Purple) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), Purple dye (If Cy5 dye is used as one of the fluorophores, internal passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available)

    40x One-step SOLIScript® Mix with SOLIScript® Reverse and Transcriptase and RiboGrip® RNase Inhibitor

    Water, nuclease free

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