PTFE Chimneys with an Aluminum Base, 384 Fountains Wash Reservoir

384 Fountain Wash Reservoir with PTFE fountains and PTFE coated aluminum base, for Cybi Well Robot with fountain and drain tubing fittings.

The VP 546 is available for use with a 384 hole (VP 540B1) blotting media support. 384 hole lint free blotting media (VP 540BC) is positioned on top of the VP 540B1 support. The wash station has a drain and a fountain outlet that can be connected to a peristaltic pump to drain the reservoir and push wash fluids through the fountains. We recommend a cleaning/wash procedure that incorporates a dip in the fountain of 99% isopropanol followed by a blot step and then another dip and blot in the same fountain.

Both the 96 and 384 wash fountains are constructed of autoclaveable PTFE and PTFE coated anodized aluminum and are designed to handle corrosive organic solvents. These fountains are designed to simulate the footprint and locating holes of CyBio’s Cybi Well microplate carrier (146 mm long by 104 mm wide and 22 mm tall). The blotting media supports (VP 540A and VP 540B1) are constructed using the autoclaveable epoxy composite G10.

Pins are washed by slowly dipping them into the fountain holes and letting the isopropanol carry the residual material away. The fountain holes are drilled through an angled surface so the effluent from each hole goes into gutters in between the column of fountains and the effluents are not able to mix until they are in the gutters. This prevents the effluents of one pin from contaminating its neighbor.